Mouse CXCL4/PF4 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse CXCL4/PF4 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse PF4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PF4 in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse PF4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse P...F4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse PF4.
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• Specificity: Natural and recombinant mouse PF4
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Pf4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CXCL4/PF4 Quantikine ELISA Kit

• chemokine (C-X-C motif) ligand 4
• C-X-C motif chemokine 4
• CXCL4
• CXCL4iroplact
• Iroplact
• MGC138298
• Oncostatin-A
• PF4
• PF-4
• platelet factor 4
• SCYB4oncostatin-A

Background

CXCL4/PF4 is a secreted chemokine that is expressed by megakaryocytes and restrains the coagulation cascade. It is released by activated platelets and regulates Thrombin/Thrombomodulin complexes, activated Protein C (APC), Coagulation Factor Xa, and Fibrin. The anticoagulant heparin neutralizes CXCL4 procoagulant effects. Complexes of heparin and CXCL4 trigger antibody formation that causes the pathological syndrome HITT. Immunogenic complexes of CXCL4 with Apolipoprotein H contribute to antiphospholipid syndrome (APS). CXCL4 interferes with the proliferative and angiogenic functions of FGF-2 and VEGF. However, it can also exert proinflammatory and pro-atherogenic effects on monocytes, macrophages, and endothelial cells. CXCL4 may signal through CXCR3B in humans.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL4/PF4 (MCX400)(9)

Publications using MCX400

• Gaur, KK;Asuru, TR;Srivastava, M;Singh, N;Purushotham, N;Poojary, B;Das, B;Bhattacharyya, S;Asthana, S;Guchhait, P; 7D, a small molecule inhibits dengue infection by increasing interferons and neutralizing-antibodies via CXCL4:CXCR3:p38:IRF3 and Sirt1:STAT3 axes respectively EMBO molecular medicine 2024-09-16 [PMID: 39284947] (Mouse)
• Luis, TC;Barkas, N;Carrelha, J;Giustacchini, A;Mazzi, S;Norfo, R;Wu, B;Aliouat, A;Guerrero, JA;Rodriguez-Meira, A;Bouriez-Jones, T;Macaulay, IC;Jasztal, M;Zhu, G;Ni, H;Robson, MJ;Blakely, RD;Mead, AJ;Nerlov, C;Ghevaert, C;Jacobsen, SEW; Perivascular niche cells sense thrombocytopenia and activate hematopoietic stem cells in an IL-1 dependent manner Nature communications 2023-09-28 [PMID: 37770432] (Mouse)
• Schroer, AB;Ventura, PB;Sucharov, J;Misra, R;Chui, MKK;Bieri, G;Horowitz, AM;Smith, LK;Encabo, K;Tenggara, I;Couthouis, J;Gross, JD;Chan, JM;Luke, A;Villeda, SA; Platelet factors attenuate inflammation and rescue cognition in ageing Nature 2023-08-16 [PMID: 37587343] (Mouse)
• Graca, FA;Stephan, A;Minden-Birkenmaier, BA;Shirinifard, A;Wang, YD;Demontis, F;Labelle, M; Platelet-derived chemokines promote skeletal muscle regeneration by guiding neutrophil recruitment to injured muscles Nature communications 2023-05-22 [PMID: 37217480] (Mouse)
• SY Heazlewood, T Ahmad, B Cao, H Cao, M Domingues, X Sun, CK Heazlewood, S Li, B Williams, M Fulton, JF White, T Nebl, CM Nefzger, JM Polo, BT Kile, F Kraus, MT Ryan, YB Sun, PFM Choong, SL Ellis, ML Anko, SK Nilsson High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production Nature Communications, 2023-04-13;14(1):2099. 2023-04-13 [PMID: 37055407] (Mouse)
• R Joseph, R Soundarara, S Vasaikar, F Yang, KL Allton, L Tian, P den Hollan, S Isgandarov, M Haemmerle, B Mino, T Zhou, C Shin, M Martinez-P, AA Sahin, J Rodriguez-, J Gelovani, JT Chang, G Acharya, AK Sood, II Wistuba, DL Gibbons, LM Solis, MC Barton, N Varadaraja, JM Rosen, XH Zhang, SA Mani CD8+ T cells inhibit metastasis and CXCL4 regulates its function British Journal of Cancer, 2021-04-01;0(0):. 2021-04-01 [PMID: 33795809] (Mouse)
• W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling Sci Rep, 2017-08-21;7(1):8402. 2017-08-21 [PMID: 28827748] (Mouse)
• Zhi H, Dai J, Liu J, Zhu J, Newman D, Gao C, Newman P Platelet Activation and Thrombus Formation over IgG Immune Complexes Requires Integrin alphaIIbbeta3 and Lyn Kinase. PLoS ONE, 2015-08-20;10(8):e0135738. 2015-08-20 [PMID: 26291522] (Mouse)
• Zheng Y, Adams T, Zhi H, Yu M, Wen R, Newman P, Wang D, Newman D Restoration of responsiveness of phospholipase Cgamma2-deficient platelets by enforced expression of phospholipase Cgamma1. PLoS ONE, 2015-03-20;10(3):e0119739. 2015-03-20 [PMID: 25793864] (Mouse)

FAQs for CXCL4/PF4 (MCX400). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Pf4