Mouse Angiopoietin-like 3 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Angiopoietin-like 3 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse ANGPT-L3 immunoassay is a 4.5 hour solid phase ELISA designed to measure ANGPT-L3 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ANGPT-L3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural ANGPT-L3 showed linear curves that were parallel to the standard curves obtained ...using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ANGPT-L3.
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• Specificity: Natural and recombinant mouse ANGPT-L3. This kit detects full-length and 27 kDa N-terminal cleaved mouse ANGPT-L3 but does not detect the 38 kDa C-terminal cleavage fragment.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Angptl3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Angiopoietin-like 3 Quantikine ELISA Kit

• AGNPT5
• Ang-5
• angiopoietin 5
• Angiopoietin-5
• angiopoietin-like 3
• Angiopoietin-like Protein 3
• angiopoietin-related protein 3
• ANGPT5angiopoietin-5
• ANGPTL3
• FHBL2

Background

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Angiopoietin-like Protein 3/ANGPTL3 (MANL30)(5)

Publications using MANL30

• Zhao, M;Linde-Garelli, KY;Zhang, Z;Toomer, D;Reghupaty, SC;Jimenez, JI;Coassolo, L;Wat, LW;Fernandez, D;Svensson, KJ; ANGPTL3 orchestrates hepatic fructose sensing and metabolism Cell reports 2025-07-09 [PMID: 40638391] (Transgenic Mouse)
• Yu, J;Jin, J;Kwon, E;Jang, H;Choi, SK;Kim, D;Kim, C;Son, S;Yoon, KJ;Heo, WD; Programmable RNA acetylation with CRISPR-Cas13 Nature chemical biology 2025-06-02 [PMID: 40456962] (Mouse)
• Zuo, Y;Zhang, C;Zhou, Y;Li, H;Xiao, W;Herzog, RW;Xu, J;Zhang, J;Chen, YE;Han, R; Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice Cell & bioscience 2023-06-15 [PMID: 37322547] (Mouse)
• ES Selen, J Choi, MJ Wolfgang Discordant hepatic fatty acid oxidation and triglyceride hydrolysis leads to liver disease JCI Insight, 2021-01-25;6(2):. 2021-01-25 [PMID: 33491665] (Transgenic Mouse)
• AC Chadwick, NH Evitt, W Lv, K Musunuru Reduced Blood Lipid Levels With In Vivo CRISPR-Cas9 Base Editing of ANGPTL3 Circulation, 2018-02-27;137(9):975-977. 2018-02-27 [PMID: 29483174] (Mouse)

FAQs for Angiopoietin-like Protein 3/ANGPTL3 (MANL30). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Angptl3