Monoamine Oxidase B Antibody

Images

 
Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Western blot analysis at 1 ug/ml in Human Brain lysate (35ug protein in RIPA buffer).
Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - (0.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA: Monoamine Oxidase B Antibody [NB100-2826] - (0.5ug/ml) as the reporter with capture rabbit antibody (2.5ug/ml).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PEP-ELISA, S-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Monoamine Oxidase B Antibody Summary

Immunogen
Peptide with sequence C-HKARKLARLTKEE corresponding to internal region according to NP_000889.3.
Specificity
This antibody is not expected to cross-react with MAOA.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAOB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • ELISA 1:100 - 1:2000
  • Peptide ELISA Detection limit 1:32000
  • Sandwich ELISA 0.5 - 1 ug/ml
Application Notes
EIA: Sandwich ELISA with increasing amount of recombinant MAOB captured by a rabbit antibody. WB: Approx. 60 kDa band observed in human brain (hippocampus) lysates (calculated MW of 58.8 kDa band according to NP_000889.3).
Positive Control
Monoamine Oxidase B Lysate (NBL1-12842)
Brain Lysate (NB820-59177)
Publications
Read Publication using NB100-2826.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Monoamine Oxidase B Antibody

  • adrenalin oxidase
  • amine oxidase [flavin-containing] B
  • EC 1.4.3
  • EC 1.4.3.4
  • MAO, brain
  • MAO, platelet
  • MAO-B
  • MGC26382
  • monoamine oxidase B
  • Monoamine oxidase type B
  • tyramine oxidase

Background

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAOB
Entrez
Uniprot