Monoamine Oxidase B Antibody

Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NB100-2826] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: Monoamine Oxidase B Antibody [NB100-2826] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Human Liver (A) with antibody at 0.1 ug/mL and Testes (B) and Breast Cancer (C) lysate with antibody at 0.3ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Monoamine Oxidase B Antibody Summary

• Description: Novus Biologicals Goat Monoamine Oxidase B Antibody (NB100-2826) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Monoamine Oxidase B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HKARKLARLTKEE corresponding to internal region according to NP_000889.3.
• Specificity: This antibody is not expected to cross-react with MAOA.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MAOB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: Western blot: Approx 60 kDa band observed in Human and Mouse Liver lysates and approx. 55-60 kDa in Human Testis and Breast cancer lysates (calculated MW of 58.8 kDa according to NP_000889.3).
• Control:

  Monoamine Oxidase B Overexpression Lysate
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Monoamine Oxidase B Antibody

• adrenalin oxidase
• amine oxidase [flavin-containing] B
• EC 1.4.3
• EC 1.4.3.21
• EC 1.4.3.4
• MAO, brain
• MAO, platelet
• MAOB
• MAO-B
• MGC26382
• Monoamine Oxidase B
• Monoamine oxidase type B
• tyramine oxidase

Background

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Monoamine Oxidase B Antibody (NB100-2826)(1)

Publication using NB100-2826

• Domschke K, Sheehan K, Lowe N et al. Association analysis of the monoamine oxidase A and B genes with attention deficit hyperactivity disorder (ADHD) in an Irish sample: Preferential transmission of the MAO-A 941G allele to affected children. Am J Med Genet B Neuropsychiatr Genet 2005-04-05 [PMID: 15717295]

Bioinformatics

• Gene Symbol: MAOB
• Entrez:
  • 4129(Human)
  • 109731(Mouse)
• Uniprot:
  • NP_000889.3(Human)