MMR/CD206/Mannose Receptor Antibody [Allophycocyanin]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse MMR/CD206 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2535A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

MMR/CD206/Mannose Receptor Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
  Leu19-Ala1388
  Accession # Q2HZ94
• Specificity: Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human MMR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MRC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody [Allophycocyanin]

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca²⁺-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 
4. Harris, N. et al. (1992) Blood 80:2363.
5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMR/CD206/Mannose Receptor Antibody (FAB2535A)(5)

Publications using FAB2535A

• Lima, C;Andrade-Barros, AI;Carvalho, FF;Falc�o, MAP;Lopes-Ferreira, M; Inflammasome Coordinates Senescent Chronic Wound Induced by Thalassophryne nattereri Venom International journal of molecular sciences 2023-05-08 [PMID: 37176162] (Flow Cytometry, Mouse)
• Yue Wang, Ziwen Cai, Gaofeng Zhan, Xing Li, Shan Li, Xuan Wang et al. Caffeic Acid Phenethyl Ester Suppresses Oxidative Stress and Regulates M1/M2 Microglia Polarization via Sirt6/Nrf2 Pathway to Mitigate Cognitive Impairment in Aged Mice following Anesthesia and Surgery Antioxidants (Basel) 3/13/2023 [PMID: 36978961]
• JW Opzoomer, JE Anstee, I Dean, EJ Hill, I Bouybayoun, J Caron, T Muliaditan, P Gordon, D Sosnowska, R Nuamah, SE Pinder, T Ng, F Dazzi, S Kordasti, DR Withers, T Lawrence, JN Arnold Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression Science Advances, 2021-11-03;7(45):eabg9518. 11/3/2021 [PMID: 34730997]
• P Salvador, DC Macías-Cej, L Gisbert-Fe, C Hernández, D Bernardo, R Alós, F Navarro-Vi, JV Esplugues, D Ortiz-Masi, MD Barrachina, S Calatayud CD16+ Macrophages Mediate Fibrosis in Inflammatory Bowel Disease J Crohns Colitis, 2018-04-27;0(0):. 2018-04-27 [PMID: 29304229] (Flow Cytometry, Mouse)
• JA Hensel, V Khattar, R Ashton, C Lee, GP Siegal, S Ponnazhaga Location of tumor affects local and distant immune cell type and number Immun Inflamm Dis, 2017-02-23;5(1):85-94. 2/23/2017 [PMID: 28250928]

Reviews for MMR/CD206/Mannose Receptor Antibody (FAB2535A) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: James Xiao IHC-Fr Mouse 05/01/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Lung tissue
• Species: Mouse
• Lot: ACRR0118031

Comments

• Comments: Antibody dilution: 1:100 in a serum-free blocking buffer (DAKO); Some non-specific bindings were detected in RBCs

reviewed by: James Xiao Flow Mouse 04/27/2018

Summary

• Application: Flow Cytometry
• Sample Tested: lung
• Species: Mouse
• Lot: ACRR0118031

Comments

• Comments: A single cell suspension of a mouse lung was blocked with Fc block for 30 minutes. Cells were then stained with the Mouse MMR/CD206 APC-conjugated Antibody (1:100 ug) in FACS buffer for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry.

Bioinformatics

• Gene Symbol: MRC1
• Uniprot:
  • Q2HZ94()