MMR/CD206/Mannose Receptor Antibody (857615) [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: Flow
• Clone: 857615
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

MMR/CD206/Mannose Receptor Antibody (857615) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MMR
  Leu19-Ala1388
  Accession # Q61830
• Specificity: Detects mouse MMR in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: MRC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody (857615) [Alexa Fluor® 350]

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) mouse CD206 precursor contains a signal sequence (19 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The mouse CD206 ECD shares 96% aa sequence identity with rat MR, and 83‑84% with human, equine, porcine and canine CD206. The cysteine-rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewis^a and ‑Lewis^x (1, 7, 9). The FNII domain mediates Ca2⁺‑independent binding of collagens (2, 10). The CTLDs participate in Ca2⁺‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin-coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
3. Harris, N. et al. (1992) Blood 80:2363.
4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.
6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRC1
• Uniprot:
  • Q61830()