MMP-9 Antibody

Images

 
Western Blot: MMP-9 Antibody [NBP1-57940] - Western blot of MMP-9. Image from verified customer review.
Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-57940] - Human prostate cancer was detected using HRP/AEC red color stain. Dilution: 2-15 ug/mL.
Western Blot: MMP-9 Antibody [NBP1-57940] - Analysis of MMP-9 in mouse brain tumor lysate (GL261 implanted mouse glioma tissue, 40 ug/lane) using anti-MMP-9 antibody. Image from verified customer review.
Western Blot: MMP-9 Antibody [NBP1-57940] - HeLa cell lysate. Antibody at 1.25 ug/mL.
Western Blot: MMP-9 Antibody [NBP1-57940] - Human Fetal Lung. Antibody at 1.0 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MMP-9 Antibody Summary

Immunogen
Synthetic peptide corresponding to the N-terminal of the human MMP-9 protein (between amino acids 150-200) UniProt P14780.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMP9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.25 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:250
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MMP-9 Knockout HeLa Cell Lysate
Reviewed Applications
Read 4 Reviews rated 4.3
using
NBP1-57940 in the following applications:

Publications
Read Publications using
NBP1-57940 in the following applications:

  • IHC
    1 publication
  • 4 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MMP-9 Antibody

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-9 Antibody (NBP1-57940)(7)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Sheep.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(4)
WB
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(1)
Sheep
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-57940 Applications Species
StacY MR, Best CA, Maxfield MW et al. Magnetic Resonance Imaging of Shear Stress and Wall Thickness in Tissue-Engineered Vascular Grafts. Tissue Eng Part C Methods. Jul 31 2018 [PMID: 29978768] (IHC-P, Sheep) IHC-P Sheep
De Cunto G, Lamberti A, de Santi MM et al. Middermal Elastolysis: Dermal Fibroblasts Cooperate with Inflammatory Cells to the Elastolytic Disorder Mediators Inflamm. 2017 Sep 16 [PMID: 29097850] (IHC, Human) IHC Human
Vuong TT, Ronning SB, Kolset SO, Pedersen ME. The enzyme profiles in the connective tissue attaching pin bones to the surrounding tissue is specific in farmed salmon (Salmo salar) and cod (Gadus morhua L.) Fish Physiol. Biochem. 2016 Jul 09 [PMID: 27394140] (IHC-P) IHC-P
Xu B, Guenther JF, Pociask DA et al. Promotion of Lung Tumor Growth by Interleukin-17. Am. J. Physiol. Lung Cell Mol. Physiol. 2014 Jul 18 [PMID: 25038189] (IHC-P, Mouse)

Details:
MMP9 antibody used for IHC-P on lungs of K-RasLA1 mice (C57BL/6 background) - autochthonous/lung tumor model (lung delivery of a recombinant adenovirus that expresses IL-17A). Primary dilution 1:200, ON 4C incubation, HRP-DAB detection, IHC images in Fig. 3C.
IHC-P Mouse
Bellini T, Trentini A, Manfrinato MC, Tamborino C, Volta CA, Di Foggia V, Fainardi E, Dallocchio F, Castellazzi M. Matrix metalloproteinase-9 activity detected in body fluids is the result of two different enzyme forms. J Biochem;151(5):493-499. 2012 May. [PMID: 22343748] (WB, Human) WB Human
Vaithilingam V, Quayum N, Joglekar MV et al. Effect of alginate encapsulation on the cellular transcriptome of human islets. Biomaterials. 2011 Nov [PMID: 21889795] (WB, Human) WB Human
Cengel Kurnaz S, Aksoy A, Guvenc D. Does Resveratrol Have a Protective Effect on Tympanosclerosis Formation in Experimentally Induced Otitis Media? Int Adv Otol 2 2014 (IHC-P, Rat) IHC-P Rat

Reviews for MMP-9 Antibody (NBP1-57940) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-57940:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot MMP-9 NBP1-57940
Enlarge
reviewed by:
Emily Thompson
WB Human 12/13/2019
View

Summary

ApplicationWestern Blot
Sample Testedconditioned media from human glioma cells,conditioned media from U251 human glioma cell line
SpeciesHuman
LotA-2
Western Blot MMP-9 NBP1-57940
Enlarge
reviewed by:
Emily Thompson
WB Human 05/02/2019
View

Summary

ApplicationWestern Blot
Sample Testedconcentrated conditioned media,U251 glioma cell line; concentrated conditioned media
SpeciesHuman
LotA-2
Western Blot MMP-9 NBP1-57940
Enlarge
reviewed by:
Anonymous
WB Mouse 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedGL261 implanted mouse glioma tissue
SpeciesMouse
LotA

Blocking

Blocking Details5% BSA+TBST, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:6000, overnight, 4 degrees Celsius, 5% BSA+TBST

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP conjugated
Secondary Manufacturer Cat#656120 (Life Technologies)
Secondary Concentration1:3000 in TBST+5% BSA

Details

Detection NotesECL detection, BioSpectrum Imaging System (UVP), 30 sec exposure
Western Blot MMP-9 NBP1-57940
Enlarge
reviewed by:
Anonymous
WB Mouse 07/21/2014
View

Summary

ApplicationWestern Blot
Sample TestedMouse glioma tissue
SpeciesMouse
LotQC3458

Blocking

Blocking DetailsMembrane blocked in TBST+5% non-fat dry milk for 1 hour at room temperature.

Primary Anitbody

Dilution Ratio1:600 in TBST+5% non-fat dry milk overnight at 4 degrees celsius.

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP conjugated
Secondary Manufacturer Cat#626120 (Life Technologies)
Secondary Concentration1:3000 in TBST+5% non-fat dry milk

Details

Detection NotesECL detection, BioSpectrum Imaging System (UVP), 1 min exposure, positive controls tested = mouse glioma tissue

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (NBP1-57940). (Showing 1 - 2 of 2 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)
  2. I have purchased MMP-9 NBP1-57940 and it is lyophilized. I would like to know how to dilute them for further processing.
    • Our product NBP1-57940 is provided lyophilized. We recommend reconstituting with 0.1ml of dH2O.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-57940AF350
Alexa Fluor 405 NBP1-57940AF405
Alexa Fluor 488 NBP1-57940AF488
Alexa Fluor 532 NBP1-57940AF532
Alexa Fluor 594 NBP1-57940AF594
Alexa Fluor 647 NBP1-57940AF647
Alexa Fluor 700 NBP1-57940AF700
Alexa Fluor 750 NBP1-57940AF750
Biotin NBP1-57940B
DyLight 350 NBP1-57940UV
DyLight 405 NBP1-57940V
DyLight 488 NBP1-57940G
DyLight 550 NBP1-57940R
DyLight 594 NBP1-57940DL594
DyLight 650 NBP1-57940C
DyLight 680 NBP1-57940FR
DyLight 755 NBP1-57940IR
FITC NBP1-57940F
HRP NBP1-57940H
Janelia Fluor 549 NBP1-57940JF549
Janelia Fluor 646 NBP1-57940JF646

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Bioinformatics Tool for MMP-9 Antibody (NBP1-57940)

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Emily Thompson
12/13/2019
Application: WB
Species: Human

Emily Thompson
05/02/2019
Application: WB
Species: Human

Anonymous
12/12/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MMP9
Entrez
Uniprot
COVID-19 update