MMP-9 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse spleen tissue and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) ...read more
MMP‑9 was detected in perfusion fixed frozen sections of rat brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) overnight at 4 °C. Tissue ...read more
MMP‑9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) at 5 µg/mL for 3 hours at room temperature. Cells were ...read more
Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP‑9 at approximately 155 kDa (as indicated) using 5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

MMP-9 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9
Ala20-Pro730
Accession # P41245
Specificity
Detects mouse MMP-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP-9 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP9
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot .25 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF909 in the following applications:

Publications
Read Publications using
AF909 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-9 Antibody [Unconjugated]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin, and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages, and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9
(Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-9 Antibody (AF909)(55)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 7 applications: Electron Microscopy, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Electron Microscopy
(1)
Flow Cytometry
(1)
ICC
(5)
IHC
(9)
IHC-Fr
(5)
IHC-P
(12)
Western Blot
(21)
All Applications
Filter By Species
Mouse
(54)
All Species
Showing Publications 1 - 10 of 55. Show All 55 Publications.
Publications using AF909 Applications Species
K Nakajima, M Ono, U Radovi?, S Dizdarevi?, SI Tomizawa, K Kuroha, G Naganatsu, I Hoshi, R Matsunaga, T Shirakawa, T Kurosawa, Y Miyazaki, M Seki, Y Suzuki, H Koseki, M Nakamura, T Suda, K Ohbo Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice Dis Model Mech, 2019;0(0):. 2019 [PMID: 31562139] (Western Blot, Mouse) Western Blot Mouse
H Zhang, M Liao, M Cao, Z Qiu, X Yan, Y Zhou, H Wu, Y Wang, J Zheng, J Ding, M Wang, Y Liao, X Chen ATRQ β-001 Vaccine Prevents Experimental Abdominal Aortic Aneurysms J Am Heart Assoc, 2019;8(18):e012341. 2019 [PMID: 31512549] (IHC-P, Mouse) IHC-P Mouse
Y Liu, Y Zhang, M Hu, YH Li, XH Cao Carnosic acid alleviates brain injury through NF?κB?regulated inflammation and Caspase?3?associated apoptosis in high fat?induced mouse models Mol Med Rep, 2019;20(1):495-504. 2019 [PMID: 31180544] (Western Blot, Mouse) Western Blot Mouse
Y Liu, Y Zhang, M Hu, YH Li, XH Cao Carnosic acid alleviates brain injury through NF?κB?regulated inflammation and Caspase?3?associated apoptosis in high fat?induced mouse models Mol Med Rep, 2019;20(1):495-504. 2019 [PMID: 31180544] (Western Blot, Mouse) Western Blot Mouse
KM Kniewallne, BM Foidl, C Humpel Platelets isolated from an Alzheimer mouse damage healthy cortical vessels and cause inflammation in an organotypic ex vivo brain slice model Sci Rep, 2018;8(1):15483. 2018 [PMID: 30341392] (IHC, Mouse) IHC Mouse
T Hara, PT Phuong, D Fukuda, K Yamaguchi, C Murata, S Nishimoto, S Yagi, K Kusunose, H Yamada, T Soeki, T Wakatsuki, I Imoto, M Shimabukur, M Sata Protease-Activated Receptor-2 Plays a Critical Role in Vascular Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice Circulation, 2018;0(0):. 2018 [PMID: 29700120] (IHC-Fr, Mouse) IHC-Fr Mouse
X Huang, Z Yue, J Wu, J Chen, S Wang, J Wu, L Ren, A Zhang, P Deng, K Wang, C Wu, X Ding, P Ye, J Xia MicroRNA-21 Knockout Exacerbates Angiotensin II-Induced Thoracic Aortic Aneurysm and Dissection in Mice With Abnormal Transforming Growth Factor-?-SMAD3 Signaling Arterioscler. Thromb. Vasc. Biol., 2018;0(0):. 2018 [PMID: 29519942] (IHC-P, Mouse) IHC-P Mouse
LQ Liu, HS Li, SP Nie, MY Shen, JL Hu, MY Xie Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells Int J Mol Sci, 2018;19(2):. 2018 [PMID: 29419740] (Western Blot, Mouse) Western Blot Mouse
J Vandooren, W Swinnen, E Ugarte-Ber, L Boon, D Dorst, E Martens, G Opdenakker Endotoxemia shifts neutrophils with TIMP-free gelatinase B/ MMP-9 from bone marrow to periphery and induces systematic upregulation of TIMP-1 Haematologica, 2017;0(0):. 2017 [PMID: 28775117] (IHC-P, Mouse) IHC-P Mouse
ND Thiele, JW Wirth, D Steins, AC Koop, H Ittrich, AW Lohse, J Kluwe TIMP-1 is upregulated, but not essential in hepatic fibrogenesis and carcinogenesis in mice Sci Rep, 2017;7(1):714. 2017 [PMID: 28386095] (Western Blot, Mouse) Western Blot Mouse
Show All 55 Publications.

Reviews for MMP-9 Antibody (AF909) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF909:
Filter by Applications
WB
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23766088
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23766088

Details

Detection NotesSee PMID 23766088

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 23766088.Note: the antibody works very good in detecting mouse osteoclast MMP9.
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/12/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23060524
SpeciesMouse
Special ApplicationsSee PMID: 23060524 for further details.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (AF909). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF909

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
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Anonymous
01/12/2015
Application: WB
Species: Mouse

 
Anonymous
01/12/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol MMP9
Uniprot
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