Rat MMP-3 ELISA Kit (Colorimetric)

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ELISA: Rat MMP-3 ELISA Kit (Colorimetric) [NBP3-06894] - Standard Curve Reference.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA, S-ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

Rat MMP-3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat MMP-3 in samples. No significant cross-reactivity or interference between Rat MMP-3 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.95
Intra-Assay
5.29
Spike Recovery
85-110 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
MMP3

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
Publications
Read Publication using NBP3-06894.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Rat MMP-3 ELISA Kit (Colorimetric)

  • CHDS6
  • EC 3.4.24
  • EC 3.4.24.17
  • matrix metallopeptidase 3 (stromelysin 1, progelatinase)
  • matrix metalloproteinase 3 (stromelysin 1, progelatinase)
  • Matrix metalloproteinase-3
  • MGC126102
  • MGC126103
  • MMP3
  • MMP-3
  • proteoglycanase
  • SL-1
  • STMY
  • STMY1MGC126104
  • STR1
  • Stromelysin 1
  • stromelysin-1
  • transin-1

Background

Effective inhibitors of matrix metalloproteinases (MMPs), a family of connective tissue-degrading enzymes, could be useful for the treatment of diseases such as cancer, multiple sclerosis, and arthritis (1). Matrix metalloproteinase-3 (MMP-3) is a protein involved in tumor invasion and metastasis. MMP3 (stromelysin) is a secreted metalloprotease with a wide range of substrate specificities. MMP3 is synthesized in a preproenzyme form with a calculated size of 54 kDa and a 17-amino acid long signal peptide. Data indicate that the expression and the possible involvement of secreted metalloproteases in tumorigenesis result from a specific interaction between the transforming factor and the target cell, which may vary in different species (2). In neural studies, matrix metalloproteinase-3 (MMP-3) was newly induced and activated in stressed dopamine cells, and the active form of MMP-3 (actMMP-3) was released into the medium. The released actMMP-3, as well as catalytically active recombinant MMP-3 (cMMP-3) led to microglial activation and superoxide generation in microglia and enhanced DA cell death. (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for MMP-3 ELISA Kit (NBP3-06894)(1)

We have publications tested in 1 application: ELISA.


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Matrix metalloproteinases (MMPs) are responsible for the degradation of extracellular matrix proteins. MMPs are essential for tissue remodeling during normal processes such as embryonic development as well as pathological conditions such as arthri...  Read full blog post.

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Bioinformatics

Gene Symbol MMP3