MMP-3 Antibody [Alexa Fluor® 532]

• Reactivity: Hu, Pm
• Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

MMP-3 Antibody [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP‑3
  Tyr18-Cys477 (Lys45Glu)
  Accession # P08254
• Specificity: Detects human and primate MMP-3 in ELISAs and Western blots. In Direct ELISA's, less than 10% cross-reactivity with recombinant human (rh) MMP‑10 is observed and less than 1% cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12, -13, and recombina
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-3 Antibody [Alexa Fluor® 532]

• CHDS6
• EC 3.4.24
• EC 3.4.24.17
• matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• matrix metalloproteinase 3 (stromelysin 1, progelatinase)
• Matrix metalloproteinase-3
• MGC126102
• MGC126103
• MMP3
• MMP-3
• proteoglycanase
• SL-1
• STMY
• STMY1MGC126104
• STR1
• Stromelysin 1
• stromelysin-1
• transin-1

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1) can degrade a broad range of substrates including collagen alpha  chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta , IGFBP-3, pro-MMP-1, pro-MMP-7, pro-MMP-8, pro-MMP-9, and pro-MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells, and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.