MMP-3 Antibody [Unconjugated]

Images

 
Western blot shows Recombinant Human MMP-3 Western Blot Standard Protein (Catalog # WBC015) and lysate of U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Goat ...read more
MMP‑3 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 10 µg/mL ...read more
MMP‑3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

MMP-3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑3
Tyr18-Cys477 (Lys45Glu)
Accession # P08254
Specificity
Detects human and primate MMP-3 in ELISAs and Western blots. In sandwich ELISAs, less than 2.5% cross-reactivity with recombinant human
(rh) MMP‑10 is observed and less than 0.1% cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12, -13, and recombinant mouse MMP‑9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human MMP-3 Antibody (Catalog # AF513)
ELISA Detection:Human MMP-3 Biotinylated Antibody (Catalog # BAF513)
Standard: Recombinant Human MMP-3 (Catalog # 513-MP)
Publications
Read Publications using
AF513 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-3 Antibody [Unconjugated]

  • CHDS6
  • EC 3.4.24
  • EC 3.4.24.17
  • matrix metallopeptidase 3 (stromelysin 1, progelatinase)
  • matrix metalloproteinase 3 (stromelysin 1, progelatinase)
  • Matrix metalloproteinase-3
  • MGC126102
  • MGC126103
  • MMP3
  • MMP-3
  • proteoglycanase
  • SL-1
  • STMY
  • STMY1MGC126104
  • STR1
  • Stromelysin 1
  • stromelysin-1
  • transin-1

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1) can degrade a broad range of substrates including collagen alpha  chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro-MMP-1, pro-MMP-7, pro-MMP-8, pro-MMP-9, and pro-MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells, and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF513
Species: Hu, Pm
Applications: WB, IHC, IP, ELISA(Cap)

Publications for MMP-3 Antibody (AF513)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA Development, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ELISA Development
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(4)
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Human
(6)
Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using AF513 Applications Species
IL-3 Decreases Cartilage Degeneration by Downregulating Matrix Metalloproteinases and Reduces Joint Destruction in Osteoarthritic Mice J Immunol, 2016;0(0):. 2016 [PMID: 27183574] (Western Blot, Mouse) Western Blot Mouse
Marina Beltrami-M, Amélie Vromman, Galina K Sukhova, Eduardo J Folco, Peter Libby Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling. PLoS ONE, 2016;0(0):1932-6203. 2016 [PMID: 27032103] (IHC-Fr, Human) IHC-Fr Human
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012;378(1):72-80. 2012 [PMID: 22366959] (ELISA Development, Human) ELISA Development Human
Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes. Life Sci., 2008;82(23):1203-9. 2008 [PMID: 18495175] (Western Blot, Human) Western Blot Human
Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes. FEBS Lett., 2007;581(9):1928-32. 2007 [PMID: 17434489] (Western Blot, Human) Western Blot Human
Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO Molecular profiling of cervical cancer progression. Br. J. Cancer, 2007;96(2):321-8. 2007 [PMID: 17242701] (IHC-P, Human) IHC-P Human
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori. Gastroenterology, 2006;130(6):1754-63. 2006 [PMID: 16697739] (Western Blot, Human) Western Blot Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF513

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Blogs on MMP-3.

MMP3 - a potential target for arthritis therapies
Matrix metalloproteinases (MMPs) are responsible for the degradation of extracellular matrix proteins. MMPs are essential for tissue remodeling during normal processes such as embryonic development as well as pathological conditions such as arthri...  Read full blog post.

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Bioinformatics

Gene Symbol MMP3
Uniprot