MMP-3 Antibody [Unconjugated]

Western blot shows Recombinant Human MMP-3 Western Blot Standard Protein (Catalog # WBC015) and lysate of U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP‑3 at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP‑3 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP‑3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Pm
• Applications: WB, IHC, IP, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP‑3
  Tyr18-Cys477 (Lys45Glu)
  Accession # P08254
• Specificity: Detects human and primate MMP-3 in ELISAs and Western blots. In sandwich ELISAs, less than 2.5% cross-reactivity with recombinant human
  (rh) MMP‑10 is observed and less than 0.1% cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12, -13, and recombinant mouse MMP‑9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MMP3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human MMP-3 Antibody (Catalog # AF513)
  ELISA Detection:Human MMP-3 Biotinylated Antibody (Catalog # BAF513)
  Standard: Recombinant Human MMP-3 (Catalog # 513-MP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-3 Antibody [Unconjugated]

• CHDS6
• EC 3.4.24
• EC 3.4.24.17
• matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• matrix metalloproteinase 3 (stromelysin 1, progelatinase)
• Matrix metalloproteinase-3
• MGC126102
• MGC126103
• MMP3
• MMP-3
• proteoglycanase
• SL-1
• STMY
• STMY1MGC126104
• STR1
• Stromelysin 1
• stromelysin-1
• transin-1

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1) can degrade a broad range of substrates including collagen alpha  chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro-MMP-1, pro-MMP-7, pro-MMP-8, pro-MMP-9, and pro-MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells, and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-3 Antibody (AF513)(7)

Publications using AF513

• IL-3 Decreases Cartilage Degeneration by Downregulating Matrix Metalloproteinases and Reduces Joint Destruction in Osteoarthritic Mice J Immunol, 2016;0(0):. 2016 [PMID: 27183574] (Western Blot, Mouse)
• Marina Beltrami-M, Amélie Vromman, Galina K Sukhova, Eduardo J Folco, Peter Libby Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling. PLoS ONE, 2016;0(0):1932-6203. 2016 [PMID: 27032103] (IHC-Fr, Human)
• Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012;378(1):72-80. 2012 [PMID: 22366959] (ELISA Development, Human)
• Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes. Life Sci., 2008;82(23):1203-9. 2008 [PMID: 18495175] (Western Blot, Human)
• Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes. FEBS Lett., 2007;581(9):1928-32. 2007 [PMID: 17434489] (Western Blot, Human)
• Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO Molecular profiling of cervical cancer progression. Br. J. Cancer, 2007;96(2):321-8. 2007 [PMID: 17242701] (IHC-P, Human)
• McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori. Gastroenterology, 2006;130(6):1754-63. 2006 [PMID: 16697739] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MMP3
• Uniprot:
  • P08254()