Equine MMP-2 ELISA Kit (Colorimetric)

• Reactivity: Eq
• Suitable Sample Type: Serum, Plasma, Tissue homogenates and other biological fluids
• Standard Curve Range: 1.56-100 ng/mL
• Sensitivity: 0.59 ng/mL

Equine MMP-2 ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 1.56-100 ng/mL
• Sensitivity: 0.59 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12
• Intra-Assay: %CV < 10
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMP2

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Equine MMP-2 ELISA Kit (Colorimetric)

• 72 kDa gelatinase
• CLG4
• CLG4A72 kDa type IV collagenase
• collagenase type IV-A
• EC 3.4.24
• EC 3.4.24.24
• Gelatinase A
• matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
• matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
• Matrix metalloproteinase-2
• matrix metalloproteinase-II
• MMP2
• MMP-2
• MMP-II
• MONA
• neutrophil gelatinase
• TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

MMP2 is a member of the Matrix Metalloproteinase (MMP) family, which are involved in extracellular matrix degradation under both normal physiological and disease processes. Specifically, MMP-2 is responsible for degrading collagens type IV, V, VII, and X and gelatin type I. Other reported functions of MMP 2 include angiogenesis, tumor invasion, tissue repair, remodeling of the vasculature, inflammation, and atherosclerotic plaque rupture. MMP-2 secretion is activated by the Ras signaling pathway. MMP2 triggers cells to begin migration by cleaving laminin-5 and exposing an integrin-binding site on the epithelial basement membrane. The cleaved of laminin has been detected in tumors and in tissues being altered, howver the activated form of MMP2 is not found in benign tumors. Thus, detection of MMP2 is a possible early indicator of tumor activity. MMP2 is most strongly expressed in normal skin fibroblasts, but may also be found in gliomas, breast and prostate tumors. Mutations in the MMP-2 gene have been linked to Torg-Winchester syndrome, Nodulosis-Arthropathy-Osteolysis (NAO) syndrome, and arthritis. Additionally, abnormal expression of matrix matalloproteinases have been associated with neoplastic traits such as loss of negative growth regulation and high invasive potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MMP2