MMP-2 Antibody - (Pro and Active)


Western Blot: MMP-2 Antibody [NB200-193] - WB analysis of recombinant pro and active forms of Human MMP-2 protein (left lane) and crude homogenate of the injured Rat peripheral nerve (right lane) using MMP-2 antibody at more
Immunocytochemistry/ Immunofluorescence: MMP-2 Antibody [NB200-193] - analysis of MMP-2 in HeLa cells using anti-MMP-2 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: MMP-2 Antibody [NB200-193] - IHC analysis of a formalin fixed paraffin embedded tissue section of human hepatocellular carcinoma using at 1:400 dilution with HRP-conjugated secondary more
Immunohistochemistry-Frozen: MMP-2 Antibody [NB200-193] - Detection of MMP2 in frozen injured rat nerve tissue section.
Immunohistochemistry-Paraffin: MMP-2 Antibody [NB200-193] - Detection of MMP-2 in paraffin-embedded human neurofibroma section.
Immunohistochemistry-Paraffin: MMP-2 Antibody [NB200-193] - IHC analysis of a formalin fixed paraffin embedded tissue section of human bladder cancer using at 1:400 dilution with HRP-conjugated secondary antibody and more

Product Details

Reactivity Hu, Mu, Rt, Ch, GPSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
1 mg/ml

MMP-2 Antibody - (Pro and Active) Summary

A synthetic peptide made to an internal region of the human MMP2 protein (between residues 400-500) [UniProt P08253]
This antibody is specific for pro and active forms of MMP2. It was tested in WB againsst recombinant mouse MMP-2, MMP3, MMP7, MMP9, and MMP16 wherein it did not react with other MMPs.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This MMP2 antibody is useful for Western Blot, Immunocytochemistry/Immunofluoresence, and Immunohistochemistry on frozen and paraffin-embedded sections. In Western Blot, bands can be seen at approximately 72kDa (proform) and 62kDa (active form). Antigen retrieval is not required for IHC. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
74 kDa
Reviewed Applications
Read 2 Reviews rated 4.5
NB200-193 in the following application:

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NB200-193 in the following applications:

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 26982283)

Alternate Names for MMP-2 Antibody - (Pro and Active)

  • 72 kDa gelatinase
  • CLG4
  • CLG4A72 kDa type IV collagenase
  • collagenase type IV-A
  • EC 3.4.24
  • EC
  • Gelatinase A
  • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
  • matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
  • Matrix metalloproteinase-2
  • matrix metalloproteinase-II
  • MMP-2 ihc
  • MMP-2 mouse
  • MMP-2 western blot
  • MMP2
  • MMP-2
  • MMP-II
  • MONA
  • neutrophil gelatinase
  • TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)


MMP2 (Matrix metalloproteinase-2, gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), is a member of the matrix metalloproteinase (MMP) enzyme family. Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP2, the signaling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-2 Antibody (NB200-193)(35)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Guinea Pig.

We have publications tested in 8 applications: Cryoculture Assay, ELISA, EM, ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Cryoculture Assay
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Guinea Pig
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Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using NB200-193 Applications Species
Deng HW, Tian Y, Zhou XJ et al. Effect of Bilberry Extract on Development of Form-Deprivation Myopia in the Guinea Pig. J Ocul Pharmacol Ther. 2016 Mar 16 [PMID:26982283] (WB, Guinea Pig) WB Guinea Pig
Achard V, Sanchez C, Tassistro V et al. Immediate Postnatal Overfeeding in Rats Programs Aortic Wall Structure Alterations and Metalloproteinases Dysregulation in Adulthood. Am J Hypertens. [PMID:26547079] (IHC-P, Rat) IHC-P Rat
Matyas C, Nemeth BT, Olah A et al. The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus. Cardiovasc Diabetol. 2015 Nov 02 [PMID:26520063] (WB, Rat) WB Rat
Tateossian H, Morse S, Simon MM et al. Interactions between the otitis media gene, Fbxo11, and p53 in the mouse embryonic lung. Dis Model Mech. [PMID:26471094] (WB, Mouse) WB Mouse
Kendziorski JA, Belcher SM. Strain-Specific Induction of Endometrial Periglandular Fibrosis in Mice Exposed During Adulthood to the Endocrine Disrupting Chemical Bisphenol A. Reprod. Toxicol. 2015 Aug 22 [PMID:26307436] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Kamekura R, Nava P, Feng M et al. Inflammation-induced desmoglein-2 ectodomain shedding compromises the mucosal barrier. Mol. Biol. Cell 2015 Jul 29 [PMID:26224314] (WB, Mouse) WB Mouse
Li PC, Sheu MJ, Ma WF et al. Anti-Restenotic Roles of Dihydroaustrasulfone Alcohol Involved in Inhibiting PDGF-BB-Stimulated Proliferation and Migration of Vascular Smooth Muscle Cells. Mar Drugs. [PMID:25988521] (WB, Rat) WB Rat
Nguyen J, Sievers R, Motion JP et al. Delivery of lipid micelles into infarcted myocardium using a lipid-linked matrix-metalloproteinase targeting peptide Mol. Pharm. 2015 Feb 02 [PMID:25642730] (IHC-Fr, Mouse) IHC-Fr Mouse
Wang Z, Xu D, Ding H et al. Loss of ATF3 promotes Akt activation and prostate cancer development in a Pten knockout mouse model. Oncogene. 2014 Dec 22 [PMID:25531328] (IHC-P, Mouse) IHC-P Mouse
Peetermans M, Vanassche T, Liesenborghs L et al. Plasminogen activation by staphylokinase enhances local spreading of S. aureus in skin infections. BMC Microbiol. 2014 Dec 17 [PMID:25515118] (WB, Mouse) WB Mouse
Show All 35 Publications.

Reviews for MMP-2 Antibody (NB200-193) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 review tested in 1 application: WB.

Reviews using NB200-193:
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Images Ratings Applications Species Date Details
reviewed by:
WB Mouse 12/23/2014


ApplicationWestern Blot
Sample TestedSee PMID 24281761


Blocking DetailsSee PMID 24281761


Detection NotesSee PMID 24281761


CommentsIndependent review from antibodyresource.comPublished in PMID 24281761.
 MMP-2 NB200-193
reviewed by:
Human 11/20/2014


Sample TestedHeLa cells


Blocking Details2% BSA in PBS, 1 h at RT

Primary Anitbody

Dilution Ratio1:500 in 2% BSA/PBS, 14h at 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#A-11008 from Life Technologies (by Molecular Probes)
Secondary Concentration0.2 ug/ml


Detection NotesZeiss LSM 780 confocal microscope, x63 oil objective
Fixation Details4% Paraformaldehyde as fixative, permeabilization with 0.2% Triton-X100
Wash Description3x washes in PBS for 5 min

Product General Protocols

View specific protocols for MMP-2 Antibody (NB200-193): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MMP-2 Antibody (NB200-193). (Showing 1 - 1 of 1 FAQs).

  1. Is this MMP2 antibody is good for mouse tissue? Are there any publications that have a mouse model using this antibody? Also does it need any antigen retrieval to do paraffin sections?
    • NB200-193 has been validated and is guaranteed for use in human, mouse, rat and chicken samples in Western blot, flow cytometry, immunocytochemistry, and immunohistochemistry in both frozen and paraffin-embedded sections. Antigen retrieval is recommended for using this antibody on paraffin-embedded sections.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB200-193AF405
Alexa Fluor (R) 488 Labeled NB200-193AF488
Alexa Fluor (R) 647 Labeled NB200-193AF647
Alexa Fluor (R) 700 Labeled NB200-193AF700
Biotin Labeled NB200-193B
DyLight 405 Labeled NB200-193V
DyLight 488 Labeled NB200-193G
DyLight 550 Labeled NB200-193R
DyLight 650 Labeled NB200-193C
FITC Labeled NB200-193F
HRP Labeled NB200-193H

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Blogs on MMP-2.

MMP-2: More Than a Cancer Marker
Matrix metalloproteinases (MMP) are a family of endopeptidases involved in the breakdown of extracellular matrix (ECM) during both normal physiological and disease processes. MMP-2 is a zinc-dependent family member that selectively cleaves collagen...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol MMP2

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