MMP-2 Antibody - (Pro and Active)

Images

 
Western Blot: MMP-2 Antibody - (Pro and Active) [NB200-193] - WB analysis of recombinant pro and active forms of Human MMP-2 protein (left lane) and crude homogenate of the injured Rat peripheral nerve (right lane) ...read more
Immunohistochemistry-Paraffin: MMP-2 Antibody - (Pro and Active) [NB200-193] - IHC analysis of a formalin fixed paraffin embedded tissue section of human hepatocellular carcinoma using at 1:400 dilution with ...read more
Immunocytochemistry/ Immunofluorescence: MMP-2 Antibody - (Pro and Active) [NB200-193] - analysis of MMP-2 in HeLa cells using anti-MMP-2 antibody. Image from verified customer review.
Immunohistochemistry-Frozen: MMP-2 Antibody - (Pro and Active) [NB200-193] - Detection of MMP2 in frozen injured rat nerve tissue section.
Immunohistochemistry-Paraffin: MMP-2 Antibody - (Pro and Active) [NB200-193] - IHC analysis of a formalin fixed paraffin embedded tissue section of human bladder cancer using at 1:400 dilution with HRP-conjugated ...read more
Western Blot: MMP-2 Antibody - (Pro and Active) [NB200-193] - Sample from Mouse vascular smooth muscle cell. Image submitted by customer review.
Immunocytochemistry/ Immunofluorescence: MMP-2 Antibody - (Pro and Active) [NB200-193] - Staining in human fibroblasts. Image from verified customer review.
Immunohistochemistry-Frozen: MMP-2 Antibody - (Pro and Active) [NB200-193] - Representative staining of MMP2 in mouse aortic sinus. This image was submitted by customer review.
Immunohistochemistry-Paraffin: MMP-2 Antibody - (Pro and Active) [NB200-193] - Analysis of paraffin embedded tissue section of human decidual cells (first trimester) using MMP-2 antibody. Image from verified customer ...read more
Immunohistochemistry-Paraffin: MMP-2 Antibody - (Pro and Active) [NB200-193] - Detection of MMP-2 in paraffin-embedded human neurofibroma section.
Immunohistochemistry-Frozen: MMP-2 Antibody - (Pro and Active) [NB200-193] - Representative staining of MMP2 in mouse aortic sinus. This image was submitted by customer review.
Simple Western: MMP-2 Antibody - (Pro and Active) [NB200-193] - Simple Western lane view shows several signals in lysates prepared from the mouse tail artery. The 72kDa and (weak) 62kDa signals are supposed to be ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, EqSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MMP-2 Antibody - (Pro and Active) Summary

Immunogen
Human C-terminal peptide (aa. 475-490).
Localization
Secreted.
Specificity
This antibody is specific for pro and active forms of MMP2. It was tested in WB againsst recombinant mouse MMP-2, MMP3, MMP7, MMP9, and MMP16 wherein it did not react with other MMPs.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Simple Western
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
This MMP2 antibody is useful for Western Blot, Immunocytochemistry/Immunofluoresence, and Immunohistochemistry on frozen and paraffin-embedded sections. In Western Blot, bands can be seen at approximately 72kDa (proform) and 62kDa (active form). Antigen retrieval is not required for IHC. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW reported in scientific literature (PMID: 28987763).
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 7 Reviews rated 4.4
using
NB200-193 in the following applications:

Publications
Read Publications using
NB200-193 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MMP-2 Antibody - (Pro and Active)

  • 72 kDa gelatinase
  • CLG4
  • CLG4A72 kDa type IV collagenase
  • collagenase type IV-A
  • EC 3.4.24
  • EC 3.4.24.24
  • Gelatinase A
  • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
  • matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
  • Matrix metalloproteinase-2
  • matrix metalloproteinase-II
  • MMP-2 ihc
  • MMP-2 mouse
  • MMP-2 western blot
  • MMP2
  • MMP-2
  • MMP-II
  • MONA
  • neutrophil gelatinase
  • TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

MMP2 (Matrix metalloproteinase-2, gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), is a member of the matrix metalloproteinase (MMP) enzyme family. Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP2, the signaling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-193
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MMP-2 Antibody (NB200-193)(42)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Guinea Pig.

We have publications tested in 9 applications: Cryoculture Assay, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
Cryoculture Assay
(1)
ELISA
(2)
EM
(1)
Flow
(1)
ICC/IF
(1)
IHC
(2)
IHC-Fr
(3)
IHC-P
(6)
WB
(22)
All Applications
Filter By Species
Human
(8)
Mouse
(16)
Rat
(8)
Chicken
(2)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using NB200-193 Applications Species
Zhang X, Chen L. Effects of CoCl2-simulated hypoxia on the expression levels of matrix metalloproteinases in renal adenocarcinoma cells and renal tubular epithelial cells Exp Ther Med Aug 1 2018 [PMID: 30116394] (WB, Human) WB Human
Nagalingam RS, Safi HA, Al-Hattab DS et al. Regulation of cardiac fibroblast MMP2 gene expression by scleraxis J. Mol. Cell. Cardiol. May 8 2018 [PMID: 29750994] (Mouse) Mouse
Wang Guangli, Zeng Yao, Chen Shaoying et al. Localization of TFPI-2 in the nucleus modulates MMP-2 gene expression in breast cancer cells. Sci Rep 2017 [PMID: 29051606] (WB, Human) WB Human
Caballero EP, Santamaria MH, Corral RS. Endogenous osteopontin induces myocardial CCL5 and MMP-2 activation that contributes to inflammation and cardiac remodeling in a mouse model of chronic Chagas heart disease. Biochim. Biophys. Acta. 2017 Oct 04 [PMID: 28987763] (Flow, Mouse) Flow Mouse
Chuanyu S, Yuqing Z, Chong X et al. Periostin promotes migration and invasion of renal cell carcinoma through the integrin/focal adhesion kinase/c-Jun N-terminal kinase pathway. Tumour Biol. Apr 1 2017 [PMID: 28381189] (WB, Human) WB Human
Kasetti RB, Phan TN, Millar JC, Zode GS. Expression of Mutant Myocilin Induces Abnormal Intracellular Accumulation of Selected Extracellular Matrix Proteins in the Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. Nov 1 2016 [PMID: 27820874] (Human) Human
Hammers DW, Sleeper MM, Forbes SC et al. Tadalafil Treatment Delays the Onset of Cardiomyopathy in Dystrophin-Deficient Hearts. J Am Heart Assoc. Aug 10 2016 [PMID: 27506543] (WB) WB
Deng HW, Tian Y, Zhou XJ et al. Effect of Bilberry Extract on Development of Form-Deprivation Myopia in the Guinea Pig. J Ocul Pharmacol Ther. 2016 Mar 16 [PMID: 26982283] (WB, Guinea Pig) WB Guinea Pig
Achard V, Sanchez C, Tassistro V et al. Immediate Postnatal Overfeeding in Rats Programs Aortic Wall Structure Alterations and Metalloproteinases Dysregulation in Adulthood. Am J Hypertens. [PMID: 26547079] (IHC-P, Rat) IHC-P Rat
Matyas C, Nemeth BT, Olah A et al. The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus. Cardiovasc Diabetol. 2015 Nov 02 [PMID: 26520063] (WB, Rat) WB Rat
Show All 42 Publications.

Reviews for MMP-2 Antibody (NB200-193) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Human, Mouse.

Reviews using NB200-193:
Filter by Applications
IF
(2)
WB
(2)
IHC-Fr
(1)
Simple Western
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(3)
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence MMP-2 NB200-193
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reviewed by:
Anonymous
IF Human 06/07/2018
View

Summary

ApplicationImmunofluorescence
Sample Tested fibroblasts
SpeciesHuman
Lot022013
Western Blot MMP-2 NB200-193
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reviewed by:
Guo Mengqi
WB Mouse 01/23/2018
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Summary

ApplicationWestern Blot
Sample Testedmouse vascular smooth muscle cell
SpeciesMouse
LotLot022013
Immunohistochemistry-Frozen MMP-2 NB200-193
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reviewed by:
Guo Mengqi
IHC-Fr Mouse 01/23/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse aortic root
SpeciesMouse
LotLot022013
Simple Western MMP-2 NB200-193
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reviewed by:
Anonymous
Simple Western Mouse 08/16/2017
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Summary

ApplicationSimple Western
Sample Testedtail artery lysate
SpeciesMouse
Lot0910-03

Comments

CommentsThis run was performed under reducing conditions using the 12-230 kDa separation system in combination with a rabbit detection system. Lysis buffer: RIPA, prot. conc.: 0.5 µg/µl.
Immunohistochemistry-Paraffin MMP-2 NB200-193
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reviewed by:
Ruhul Choudhury
IHC-P Human 01/18/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman decidua
SpeciesHuman
Lot0213-02

Comments

CommentsSodium Citrate antigen retrieval.
 
reviewed by:
Anonymous
WB Mouse 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24281761
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24281761

Details

Detection NotesSee PMID 24281761

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 24281761.
Immunofluorescence MMP-2 NB200-193
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reviewed by:
Anonymous
IF Human 11/20/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
Lot21512

Blocking

Blocking Details2% BSA in PBS, 1 h at RT

Primary Anitbody

Dilution Ratio1:500 in 2% BSA/PBS, 14h at 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#A-11008 from Life Technologies (by Molecular Probes)
Secondary Concentration0.2 ug/ml

Details

Detection NotesZeiss LSM 780 confocal microscope, x63 oil objective
Fixation Details4% Paraformaldehyde as fixative, permeabilization with 0.2% Triton-X100
Wash Description3x washes in PBS for 5 min

Product General Protocols

View specific protocols for MMP-2 Antibody (NB200-193): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MMP-2 Antibody (NB200-193). (Showing 1 - 1 of 1 FAQs).

  1. Is this MMP2 antibody is good for mouse tissue? Are there any publications that have a mouse model using this antibody? Also does it need any antigen retrieval to do paraffin sections?
    • NB200-193 has been validated and is guaranteed for use in human, mouse, rat and chicken samples in Western blot, flow cytometry, immunocytochemistry, and immunohistochemistry in both frozen and paraffin-embedded sections. Antigen retrieval is recommended for using this antibody on paraffin-embedded sections.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-193AF405
Alexa Fluor 488 Labeled NB200-193AF488
Alexa Fluor 647 Labeled NB200-193AF647
Alexa Fluor 700 Labeled NB200-193AF700
Biotin Labeled NB200-193B
DyLight 405 Labeled NB200-193V
DyLight 488 Labeled NB200-193G
DyLight 550 Labeled NB200-193R
DyLight 650 Labeled NB200-193C
FITC Labeled NB200-193F
HRP Labeled NB200-193H

Additional Array Products

Bioinformatics Tool for MMP-2 Antibody (NB200-193)

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Diseases for MMP-2 Antibody (NB200-193)

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Pathways for MMP-2 Antibody (NB200-193)

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PTMs for MMP-2 Antibody (NB200-193)

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Research Areas for MMP-2 Antibody (NB200-193)

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Blogs on MMP-2.

MMP-2: More Than a Cancer Marker
Matrix metalloproteinases (MMP) are a family of endopeptidases involved in the breakdown of extracellular matrix (ECM) during both normal physiological and disease processes. MMP-2 is a zinc-dependent family member that selectively cleaves collagen...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Recent Reviews

4.4
5
4
4
2
3
1
2
0
1
0

Anonymous
06/07/2018
Application: IF
Species: Human

Guo Mengqi
01/23/2018
Application: WB
Species: Mouse

Guo Mengqi
01/23/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol MMP2