MMP-2 Antibody - (Pro and Active) Summary
A synthetic peptide made to an internal region of the human MMP2 protein (between residues 400-500) [UniProt P08253]
This antibody is specific for pro and active forms of MMP2. It was tested in WB againsst recombinant mouse MMP-2, MMP3, MMP7, MMP9, and MMP16 wherein it did not react with other MMPs.
Immunogen affinity purified
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- Western Blot 1:1500-1:3500
- Simple Western
- Flow Cytometry
- Immunocytochemistry/Immunofluorescence 1:100-1:500
- Immunohistochemistry 2-4 ug/ml
- Immunohistochemistry-Frozen 2-4 ug/ml
- Immunohistochemistry-Paraffin 2-4 ug/ml
This MMP2 antibody is useful for Western Blot, Immunocytochemistry/Immunofluoresence, and Immunohistochemistry on frozen and paraffin-embedded sections. In Western Blot, bands can be seen at approximately 72kDa (proform) and 62kDa (active form). Antigen retrieval is not required for IHC. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW reported in scientific literature (PMID: 28987763).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Guinea Pig reactivity reported in scientific literature (PMID: 26982283)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for MMP-2 Antibody - (Pro and Active)
- 72 kDa gelatinase
- CLG4A72 kDa type IV collagenase
- collagenase type IV-A
- EC 3.4.24
- EC 188.8.131.52
- Gelatinase A
- matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
- matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
- Matrix metalloproteinase-2
- matrix metalloproteinase-II
- MMP-2 ihc
- MMP-2 mouse
- MMP-2 western blot
- neutrophil gelatinase
- TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
MMP2 (Matrix metalloproteinase-2, gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), is a member of the matrix metalloproteinase (MMP) enzyme family. Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP2, the signaling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
View specific protocols for MMP-2 Antibody (NB200-193):
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FAQs for MMP-2 Antibody (NB200-193). (Showing 1 - 1 of 1 FAQs).
Is this MMP2 antibody is good for mouse tissue? Are there any publications that have a mouse model using this antibody? Also does it need any antigen retrieval to do paraffin sections?
- NB200-193 has been validated and is guaranteed for use in human, mouse, rat and chicken samples in Western blot, flow cytometry, immunocytochemistry, and immunohistochemistry in both frozen and paraffin-embedded sections. Antigen retrieval is recommended for using this antibody on paraffin-embedded sections.
Other Available Formats
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