MMP-2 Antibody [Unconjugated]

MMP-2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse/ Rat MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

MMP2, MMP9, and RANKL expression in AAAs.A. Immunohistochemical staining for indicated proteins of serial sections of aortas one week after CaCl2 treatment. Elastic van Gieson staining is also shown. SM alpha -actin and F4/80 were stained to locate SMCs and macrophages, respectively. Shown are representative images of 4 or more samples in each group. Scale bars, 50 µm. B. Relative positive staining area of MMP2, MMP9, and RANKL in sections from control diet and EPA diet groups. n = 4–5. *P<0.05. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0096286), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MMP-2
  Ile34-Cys662
  Accession # P33434
• Specificity: Detects mouse and rat MMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human MMP-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) MMP-3 and rmMMP-9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MMP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-2 Antibody [Unconjugated]

• 72 kDa gelatinase
• CLG4
• CLG4A72 kDa type IV collagenase
• collagenase type IV-A
• EC 3.4.24
• EC 3.4.24.24
• Gelatinase A
• matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
• matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
• Matrix metalloproteinase-2
• matrix metalloproteinase-II
• MMP2
• MMP-2
• MMP-II
• MONA
• neutrophil gelatinase
• TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites. The amino acid sequences of the proenzymes are identical between mouse and rat.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-2 Antibody (AF1488)(38)

Publications using AF1488

• Kwon, HJ;Santhosh, D;Huang, Z; A novel monomeric amyloid ?-activated signaling pathway regulates brain development via inhibition of microglia eLife 2024-12-05 [PMID: 39635981] (Immunohistochemistry, Mouse)
• Wang, D;Saleem, S;Sullivan, RD;Zhao, T;Reed, GL; Differences in Acute Expression of Matrix Metalloproteinases-9, 3, and 2 Related to the Duration of Brain Ischemia and Tissue Plasminogen Activator Treatment in Experimental Stroke International journal of molecular sciences 2024-08-30 [PMID: 39273389] (Immunohistochemistry, Mouse)
• Fu Y, Liu H, Li K et al. C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysms Front Immunol 2023-09-11 [PMID: 37753091]
• Weilai Fu, Haole Liu, Panpan Wei, Congcong Xia, Qingqing Yu, Kangli Tian et al. Genetic deficiency of protein inhibitor of activated STAT3 suppresses experimental abdominal aortic aneurysms Frontiers in Cardiovascular Medicine 2023-03-15 [PMID: 37008329]
• Leng Ni, Lijuan Liu, Wanqu Zhu, Richard Telljohann, Jing Zhang, Robert E. Monticone et al. Inflammatory Role of Milk Fat Globule–Epidermal Growth Factor VIII in Age‐Associated Arterial Remodeling Journal of the American Heart Association 2022-09-06 [PMID: 36000422] (Western Blot, Transgenic Mouse)
• Liu H, Tian K, Xia C et al. Kunming mouse strain is less susceptible to elastase induced abdominal aortic aneurysms Animal Model Exp Med 3/1/2022 [PMID: 35229996]
• K Tian, C Xia, H Liu, B Xu, P Wei, W Fu, M Lu, Y Li, Y Li, D Cheng, E Liu, S Zhao Temporal and Quantitative Analysis of Aortic Immunopathologies in Elastase-Induced Mouse Abdominal Aortic Aneurysms Journal of Immunology Research, 2021-11-16;2021(0):6297332. 2021-11-16 [PMID: 34825008] (IHC, Mouse)
• C Chun, X Qi, F Wang, KB Madrid, LA Saldarriag, MR Fisch, ML Brantly, GR Upchurch, Z Jiang Nicotine Exacerbates TAAD Formation Induced by Smooth Muscle-Specific Deletion of the TGF-beta Receptor 2 Journal of Immunology Research, 2021-10-01;2021(0):6880036. 2021-10-01 [PMID: 34646889] (IHC, Mouse)
• Matthew William Grol, Nele A Haelterman, Joohyun Lim, Elda M Munivez, Marilyn Archer, David M Hudson et al. Tendon and motor phenotypes in the Crtap-/- mouse model of recessive osteogenesis imperfecta eLife 2021-05-26 [PMID: 34036937]
• Kui Li, Zheng Zhou, Ju Li, Rui Xiang miR-146b Functions as an Oncogene in Oral Squamous Cell Carcinoma by Targeting HBP1 Technol Cancer Res Treat 2020-12-17 [PMID: 33327874]
• SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm Exp. Mol. Med., 2020-09-14;0(0):. 2020-09-14 [PMID: 32929220] (Western Blot, Mouse)
• CY Lu, SC Lai, HH Lee, HT Chien, KP Lan, KM Chen Matrix metalloproteinases activation in Toxocara canis induced pulmonary pathogenesis J Microbiol Immunol Infect, 2020-08-11;0(0):. 2020-08-11 [PMID: 32826193] (IHC, Mouse)
• X Gou, Y Xue, H Zheng, G Yang, S Chen, Z Chen, G Yuan Gelatinases Cleave Dentin Sialoprotein Intracellularly Front Physiol, 2020-06-25;11(0):686. 6/25/2020 [PMID: 32670089]
• H Zhang, M Liao, M Cao, Z Qiu, X Yan, Y Zhou, H Wu, Y Wang, J Zheng, J Ding, M Wang, Y Liao, X Chen ATRQ β-001 Vaccine Prevents Experimental Abdominal Aortic Aneurysms J Am Heart Assoc, 2019-09-12;8(18):e012341. 2019-09-12 [PMID: 31512549] (IHC-P, Mouse)
• F Luo, AP Tran, L Xin, C Sanapala, BT Lang, J Silver, Y Yang Modulation of proteoglycan receptor PTP? enhances MMP-2 activity to promote recovery from multiple sclerosis Nat Commun, 2018-10-08;9(1):4126. 10/8/2018 [PMID: 30297691]
• LQ Liu, HS Li, SP Nie, MY Shen, JL Hu, MY Xie Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells Int J Mol Sci, 2018-02-08;19(2):. 2018-02-08 [PMID: 29419740] (Western Blot, Mouse)
• CL Lin, JH Jeng, CC Wu, SL Hsieh, GC Huang, W Leung, CT Lee, CY Chen, CH Lee Chemopreventive Potential of 2,3,5,4&#039;-Tetrahydroxystilbene-2-O-?-D-glucoside on the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colorectal Cancer in Rats Biomed Res Int, 2017-11-07;2017(0):3634915. 2017-11-07 [PMID: 29238715] (Western Blot, Rat)
• PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression J Hematol Oncol, 2017-01-25;10(1):33. 2017-01-25 [PMID: 28122633] (IHC-P, Human)
• Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries Physiol Rep, 2016-12-01;4(23):. 2016-12-01 [PMID: 27923978] (IHC, Mouse)
• Huey Lin, Mia Angeli, Kwang Jin Chung, Chukwuemeka Ejimadu, Angelica Rivera Rosa, Techung Lee sFRP2 activates Wnt/ beta -catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix remodeling American Journal of Physiology-Cell Physiology 2016-11-01 [PMID: 27605451] (Western Blot, Mouse)
• Zhang Yiping, He Yuanqiao, Yu Hongbo et al. Liquiritigenin Protects Rats from Carbon Tetrachloride Induced Hepatic Injury through PGC-1a Pathway. Evidence-Based Complementary and Alternative Medicine : ECam 1/1/2015 [PMID: 26199636]
• Dohi , Kenji, Kraemer , Brian C, Erickson , Michelle, McMillan , Pamela J, Kovac , Andrej, Flachbartova , Zuzana, Hansen , Kim M, Shah , Gul N, Sheibani , Nader, Salameh , Therese, Banks , William Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury. PLoS ONE, 2014-09-24;9(9):e108034. 2014-09-24 [PMID: 25251220] (Western Blot, Mouse)
• Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling. PLoS ONE, 2014-05-05;9(5):e96286. 2014-05-05 [PMID: 24798452] (IHC-P, Mouse)
• O'Neal W, Griffin W, Kent S, Faiz F, Hodges J, Vuncannon J, Virag J Deletion of the EphA2 receptor exacerbates myocardial injury and the progression of ischemic cardiomyopathy. Front Physiol, 2014-04-24;5(0):132. 2014-04-24 [PMID: 24795639] (Western Blot, Mouse)
• Salmon M, Johnston W, Woo A, Pope N, Su G, Upchurch G, Owens G, Ailawadi G KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation. Circulation, 2013-09-10;128(11):S163-74. 2013-09-10 [PMID: 24030402] (IHC-P, Mouse)
• Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J Immunol, 2013-04-15;190(10):5329-36. 2013-04-15 [PMID: 23589623] (ELISA Development (Capture), Mouse)
• Gkantidis , Nikolaos, Blumer , Susan, Katsaros , Christos, Graf , Daniel, Chiquet , Matthias Site-Specific Expression of Gelatinolytic Activity during Morphogenesis of the Secondary Palate in the Mouse Embryo. PLoS ONE, 2012-10-16;7(10):e47762. 2012-10-16 [PMID: 23091646] (IHC-Fr, Mouse)
• Lau , Wei Ling, Leaf , Elizabet, Hu , Ming Cha, Takeno , Marc M, Kuro-o , Makoto, Moe , Orson W, Giachelli , Cecilia Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet. Kidney Int, 2012-08-29;82(12):1261-70. 2012-08-29 [PMID: 22932118] (IHC, Mouse)
• Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice. J. Biol. Chem., 2012-03-23;287(20):16356-64. 2012-03-23 [PMID: 22447930] (IHC-P, Mouse)
• Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans. Diabetologia, 2011-03-25;54(6):1468-79. 2011-03-25 [PMID: 21437772] (Western Blot, Mouse)
• Pai A, Leaf EM, El-Abbadi M, Giachelli CM Elastin degradation and vascular smooth muscle cell phenotype change precede cell loss and arterial medial calcification in a uremic mouse model of chronic kidney disease. Am. J. Pathol., 2011-02-01;178(2):764-73. 2011-02-01 [PMID: 21281809] (IHC-P, Mouse)
• Lind T, Lind PM, Jacobson A, Hu L, Sundqvist A, Risteli J, Yebra-Rodriguez A, Rodriguez-Navarro A, Andersson G, Melhus H High dietary intake of retinol leads to bone marrow hypoxia and diaphyseal endosteal mineralization in rats. Bone, 2010-10-28;48(3):496-506. 2010-10-28 [PMID: 21035577] (IHC, Rat)
• John M. Hartney, Jeanette Brown, Hong Wei Chu, Ling Yi Chang, Roberta Pelanda, Raul M. Torres Arhgef1 regulates alpha5beta1 integrin-mediated matrix metalloproteinase expression and is required for homeostatic lung immunity The American Journal of Pathology 2010-03-01 [PMID: 20093499]
• Bujak M, Dobaczewski M, Chatila K, Mendoza LH, Li N, Reddy A, Frangogiannis NG Interleukin-1 receptor type I signaling critically regulates infarct healing and cardiac remodeling. Am. J. Pathol., 2008-06-05;173(1):57-67. 2008-06-05 [PMID: 18535174] (Western Blot, Mouse)
• Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse. Kidney Int., 2008-03-26;74(1):47-51. 2008-03-26 [PMID: 18368029] (Western Blot, Mouse)
• Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Blood, 2006-11-02;109(5):2234-42. 2006-11-02 [PMID: 17082320] (Western Blot, Mouse)
• Rutkowski JM, Boardman KC, Swartz MA Characterization of lymphangiogenesis in a model of adult skin regeneration. Am. J. Physiol. Heart Circ. Physiol., 2006-04-28;291(3):H1402-10. 2006-04-28 [PMID: 16648194] (ICC, Mouse)
• Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice. Infect. Immun., 2005-10-01;73(10):6962-73. 2005-10-01 [PMID: 16177376] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: MMP2
• Uniprot:
  • P33434()