MMP-15/MT2-MMP Antibody (MM0028-5D11)

Images

 
Immunohistochemistry-Paraffin: MMP15 Antibody (MM0028-5D11) [NB110-60994] - Human placenta (formalin fixed and paraffin embedded) section is subjected to IHC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
MM0028-5D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

MMP-15/MT2-MMP Antibody (MM0028-5D11) Summary

Immunogen
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified recombinant human matrix metalloproteinase-15.
Specificity
MMP15 (MM0028-5D11)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP15
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400-1:1000
  • Immunohistochemistry 1:50-200
  • Immunohistochemistry-Paraffin 1:50-200
Application Notes
This antibody recognizes human MMP-15 in western blot without cross reactivity with human MMP-14/16.
Positive Control
MMP-15/MT2-MMP Lysate (NBL1-13154)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for MMP-15/MT2-MMP Antibody (MM0028-5D11)

  • EC 3.4.24
  • EC 3.4.24.-
  • EC 3.4.24.80
  • matrix metallopeptidase 15 (membrane-inserted)
  • matrix metalloproteinase 15 (membrane-inserted)
  • Membrane-type matrix metalloproteinase 2
  • Membrane-type-2 matrix metalloproteinase
  • MMP15
  • MMP-15
  • MT2-MMP
  • MT2-MMPMT2MMP
  • MTMMP2MT-MMP 2
  • SMCP-2matrix metalloproteinase-15

Background

The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins. MMP15, first described as MTMMP2, is a metalloproteinase with a type I transmembrane domain. Initially reported in lung tissue, MMP15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP15 is usually found in much lower levels than the more ubiquitous MMP14 (MT1MMP). MMP15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MMP15
Entrez