MMP-15/MT2-MMP Overexpression Lysate (Native)

Images

 
Western Blot: MMP15 Overexpression Lysate (Adult Normal) [NBL1-13154] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MMP15.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MMP-15/MT2-MMP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207318 and based on accession number NM_002428. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens matrix metallopeptidase 15 (membrane-inserted) (MMP15), mRNA.
Gene
MMP15

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MMP-15/MT2-MMP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MMP-15/MT2-MMP Overexpression Lysate (Native)

  • EC 3.4.24
  • EC 3.4.24.-
  • EC 3.4.24.80
  • matrix metallopeptidase 15 (membrane-inserted)
  • matrix metalloproteinase 15 (membrane-inserted)
  • Membrane-type matrix metalloproteinase 2
  • Membrane-type-2 matrix metalloproteinase
  • MMP15
  • MMP-15
  • MT2-MMP
  • MT2-MMPMT2MMP
  • MTMMP2MT-MMP 2
  • SMCP-2matrix metalloproteinase-15

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MMP-15/MT2-MMP Lysate (NBL1-13154) (0)

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Bioinformatics

Gene Symbol MMP15
Entrez