Recombinant Human MMP-17 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human MMP-17 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003). The specific activity is >150 pmol/min/μg, as measured under the described condition.
Mouse myeloma cell line, NS0-derived human MMP-17 protein
Ala39-Ser531 (Arg125Pro) & (Ala182Thr), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Enzymes
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane


  • Enzyme Activity
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
56-65 kDa, reducing conditions
Read Publications using
7796-MP in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in MES, NaCl, CaCl2 and Glycerol.
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Assay Procedure
  • Activation Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5 (TCNB) + 100 µM ZnCl2
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, pH 7.5
  • Recombinant Human MMP-17 (rhMMP-17) (Catalog # 7796-MP)
  • p-aminophenylmercuric acetate (APMA) (Sigma, Catalog # A-9563), 100 mM stock in DMSO
  • Substrate: MCA-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003), 2 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: Gemini EM by Molecular Devices) or equivalent
  1. Activate rhMMP-17 at 100 µg/mL with 1 mM APMA in Activation Buffer.
  2. Incubate at room temperature for 2 hours.
  3. Dilute activated rhMMP-17 to 2 ng/µL in Assay Buffer.
  4. Dilute Substrate to 20 µM in Assay Buffer.
  5. Load 50 µL of the 2 ng/µL rhMMP-17 into plate, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate without any rhMMP‑17.
  6. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:
  • rhMMP-17: 0.1 µg
  • Substrate: 10 µM


Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MMP-17 Protein, CF

  • matrix metallopeptidase 17 (membrane-inserted)
  • matrix metalloproteinase 17 (membrane-inserted)
  • matrix metalloproteinase-17
  • membrane-type matrix metalloproteinase 4
  • membrane-type-4 matrix metalloproteinase
  • MMP17
  • MMP-17
  • MT4MMP
  • MT4-MMP
  • MT-MMP 4
  • MTMMP4


Matrix metalloproteinase 17 (MMP‑17) is a zinc metalloproteinase that is an integral membrane protein (1). It belongs to the membrane‑type MMP (MT‑MMP) subfamily, and is also known as MT4‑MMP. It is associated with the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (1). It has the potential to function as a pro‑tumor necrosis factor‑a  (TNF‑a) convertase (2). Unlike MMP‑14 (MT1‑MMP), it is a poor activator of pro‑MMP‑2. MMP‑17 is relatively poor at digesting components of the extracellular matrix, but does cleave fibrinogen and fibrin (2). MMP‑17 is known to activate the aggrecanase ADAMTS‑4 through proteolysis (3). MMP‑17 is expressed in many tissues with the highest levels seen in the brain, colon, ovary, testis, and kidney papilla (4). It has been detected in several human cancers, including gliomas, prostate carcinomas, and breast carcinomas (5). Recombinant Human MMP‑17 was expressed without its GPI anchoring sequence, resulting in its secretion as a soluble protein.
  1. Itoh, Y. et al. (1999) J. Biol. Chem. 274:34260.
  2. English, W. R. et al. (2000) J. Biol. Chem. 275:14046.
  3. Gao, G. et al. (2004) J. Biol. Chem. 279:10042.
  4. Srichai, M. B. et al. (2011) PLoS ONE 6:e17099.
  5. Sohail, A. et al. (2008) Cancer Metastasis Rev.  27:289.

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Gene Symbol MMP17