MMP-11 Antibody (SN74-08)

Western Blot: MMP-11 Antibody (SN74-08) [NBP2-67670] - Analysis of MMP11 on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670] - Staining MMP11 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670] - Analysis of paraffin-embedded mouse spleen tissue using anti-MMP11 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670] - Staining MMP11 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670] - Staining MMP11 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP11 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670] - Analysis of paraffin-embedded human spleen tissue using anti-MMP11 antibody. Counter stained with hematoxylin.

Product Discontinued

MMP-11 Antibody (SN74-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MMP-11 aa 439-488 / 488. (SwissProt: P24347 Human; SwissProt: Q02853 Mouse; SwissProt: Q499S5 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MMP11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MMP-11 Antibody (SN74-08)

• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.24
• matrix metallopeptidase 11 (stromelysin 3)
• matrix metalloproteinase 11 (stromelysin 3)
• Matrix metalloproteinase-11
• MMP11
• MMP-11
• SL-3
• ST3
• STMY3stromelysin 3
• stromelysin III
• stromelysin-3

Background

The matrix metalloproteinases are a tightly regulated family of enzymes that degrade extracellular matrix and basement membrane components. Recent evidence suggests that these proteases and their specific inhibitors play important roles in normal developmental processes and in pathological conditions (1). MMP-11 (stromelysin-3), a new member of the matrix metalloproteinase family, is expressed in tissues undergoing the active remodeling associated with embryonic development, wound healing and tumor invasion. But like all other members of the matrix metalloproteinase gene family, stromelysin-3 is synthesized as an inactive precursor that must be processed to its mature form in order to express enzymic activity (2). MMP-11, like an inflammatory mediator, may exert a chemotactic influence on macrophages which aggregate in the mesangium; MMP-11 is not likely to have a parallel mitogenic or antifibrotic effect in diseased glomeruli (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-11 Antibody (NBP2-67670)(5)

Publications using NBP2-67670

• Ahn H, Song GJ, Jang SH et al. Predicting the Recurrence of Gastric Cancer Using the Textural Features of Perigastric Adipose Tissue on [18F]FDG PET/CT International journal of molecular sciences 2022-10-09 [PMID: 36233285] (IHC-P, Human)
• Ahn H, Song GJ, Jang SH et al. Relationship of FDG PET/CT Textural Features with the Tumor Microenvironment and Recurrence Risks in Patients with Advanced Gastric Cancers Cancers 2022-08-15 [PMID: 36010928] (IHC-P, Human)
• Kim DJ, Iwasaki A, Chien AL, Kang S UVB-mediated DNA damage induces matrix metalloproteinases to promote photoaging in an AhR- and SP1-dependent manner JCI insight 2022-03-22 [PMID: 35316219]
• Ahn H, Won Lee J, Jang SH Et al. Prognostic significance of imaging features of peritumoral adipose tissue in FDG PET/CT of patients with colorectal cancer European journal of radiology 2021-11-17 [PMID: 34801879] (IHC-P, Human)
• Wang Y, Hu S, Yao G, Sun Y Identification of HOXA10 target genes in human endometrial stromal cells by RNA-seq analysis Acta biochimica et biophysica Sinica 2021-01-19 [PMID: 33462609] (WB, Mouse)

Bioinformatics

• Gene Symbol: MMP11