MLX Antibody (PCRP-MLX-1G8) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: MLX Antibody (PCRP-MLX-1G8) [NBP3-08241] - Immunofluorescence Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti- Mouse IgG-CF488 (green); counterstain phalloidin (red).

Flow Cytometry: MLX Antibody (PCRP-MLX-1G8) [NBP3-08241] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: MLX Antibody (PCRP-MLX-1G8) [NBP3-08241] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, MA
• Clone: PCRP-MLX-1G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MLX Antibody (PCRP-MLX-1G8) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07337).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human MLX protein (Uniprot: Q9UH92)
• Localization: Nucleus and cytoplasm.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLX
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for MLX Antibody (PCRP-MLX-1G8) - Azide and BSA Free

• BHLHD13
• bHLHd13Max-like bHLHZip protein
• BigMax alpha
• BigMax protein
• Class D basic helix-loop-helix protein 13
• MAD7
• MAX-like protein X
• MLX
• MXD7
• TCFL4
• TCFL4Protein BigMax
• transcription factor-like 4
• Transcription factor-like protein 4

Background

Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad 1, Mad 3, Mad 4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad 1, Mad 4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLX