MLF1 Interacting Protein [p Thr78] Antibody

Immunohistochemistry-Paraffin: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-02440] - Staining of human colon adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Product Discontinued

MLF1 Interacting Protein [p Thr78] Antibody Summary

• Immunogen: Synthetic peptide from human MLF1 Interacting Protein phosphoylated at Thr78.
• Modification: p Thr78
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MLF1IP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:25000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MLF1 Interacting Protein [p Thr78] Antibody

• CENP-50
• CENP-U
• CENPU50
• CENPUMLF1-interacting protein
• centromere protein of 50 kDa
• centromere protein U
• FLJ23468
• ICEN24
• Interphase centromere complex protein 24
• KLIP1CENP50
• KSHV latent nuclear antigen interacting protein 1
• KSHV latent nuclear antigen-interacting protein 1
• MLF1 interacting protein
• PBIP1
• Polo-box-interacting protein 1

Background

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLF1IP
• Entrez:
  • 79682(Human)
  • 71876(Mouse)
  • 306464(Rat)
• Uniprot:
  • Q71F23(Human)