MKK7/MEK7 [p Thr277] Antibody - BSA Free

Western Blot: MKK7/MEK7 [p Thr277] Antibody [NBP2-29678] - (No peptide/Lane 1), A431 treated with Calyculin A and okadaic acid (Control peptide/Lane 2), A431 treated with Calyculin A and okadaic acid (Phosphopeptide (Thr275/Ser277)/Lane 3) lysates were probed Anti-Phospho-MKK7/SAPKK4 (Thr275/Ser277) (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Phospho-MKK7/SAPKK4 (Thr275/Ser277) (~47 kDa).

Western Blot: MEK7 [p Thr277] Antibody [NBP2-29678] - Recombinant MKK7b1 (14-542) was left untreated (Lane 1) or phosphorylated by MEKK1 (14-196) (Lane 2) and probed with anti-phospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution).

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MKK7/MEK7 [p Thr277] Antibody - BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_145185.2
• Immunogen: peptide corresponding to amino acids surrounding Thr275/Ser277 of human MKK7/SKK4.
• Modification: p Thr277
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP2K7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (phH 7.4), 0.15M NaCl and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Relevant Entrez Gene Numbers: NM_145185.2

Alternate Names for MKK7/MEK7 [p Thr277] Antibody - BSA Free

• c-Jun N-terminal kinase kinase 2
• dual specificity mitogen-activated protein kinase kinase 7
• EC 2.7.12.2
• JNK kinase 2
• JNK-activating kinase 2
• JNKK 2
• JNKK2
• MAP kinase kinase 7
• MAP2K7
• MAPK/ERK kinase 7
• MAPKK7
• MEK 7
• MEK7
• mitogen-activated protein kinase kinase 7
• MKE7
• MKK7
• MKK7MAPKK 7
• PRKMK7

Background

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K7
• Uniprot:
  • O14733()