MKK7/MEK7 Antibodies

HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="MKK7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human MKK7 Monoclonal Antibody (Catalog # MAB3579) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />