MKK3/MEK3 [p Ser189] Antibody - BSA Free

Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP) NB100-82048

Immunocytochemistry/Immunofluorescence: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Staining of methanol-fixed Hela cells using NB100-82048

Western Blot: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) antibody

Immunohistochemistry-Paraffin: MKK3/MEK3 [p Ser189] Antibody [NB100-82048] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using NB100-82048(left) or the same antibody preincubated with blocking peptide (right).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MKK3/MEK3 [p Ser189] Antibody - BSA Free Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MKK3/MEK3 around the phosphorylation site of serine 189 (V-D-SP-V-A).
• Modification: p Ser189
• Specificity: Detects endogenous levels of MKK3/MEK3 only when phosphorylated at serine189.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP2K3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100~1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:1000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  MKK3/MEK3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MKK3/MEK3 [p Ser189] Antibody - BSA Free

• MAP kinase kinase 3
• MAP2K3
• MAPK/ERK kinase 3
• MAPKK 3
• MAPKK3PRKMK3dual specificity mitogen-activated protein kinase kinase 3
• MEK3
• MEK3MEK 3
• mitogen activated protein kinase kinase 3
• mitogen-activated protein kinase kinase 3
• MKK3
• MKK3EC 2.7.12.2
• PRKMK3

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKK3/MEK3 Antibody (NB100-82048)(1)

Publication using NB100-82048

• Mannam P, Shinn AS, Srivastava A et al. MKK3 regulates mitochondrial biogenesis and mitophagy in sepsis-induced lung injury Am J Physiol Lung Cell Mol Physiol. 2014-01-30 [PMID: 24487387] (WB, Human)

Bioinformatics

• Gene Symbol: MAP2K3
• Entrez:
  • 5606(Human)
  • 303200(Rat)
• Uniprot:
  • P46734()