MKK7/MEK7 Antibody (4E5) - BSA Free

Images

 
Western Blot: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Western blot analysis using MAP2K7 mAb against human MAP2K7 (AA: 7-178) recombinant protein. (Expected MW is 45.1 kDa)
Immunocytochemistry/ Immunofluorescence: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunofluorescence analysis of Hela cells using MAP2K7 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 ...read more
Immunohistochemistry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K7 mouse mAb with DAB staining.
Flow Cytometry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Flow cytometric analysis of Hela cells using MAP2K7 mouse mAb (green) and negative control (red).
Immunohistochemistry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MAP2K7 mouse mAb with DAB staining.
ELISA: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

MKK7/MEK7 Antibody (4E5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human MKK7/MEK7 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP2K7
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for MKK7/MEK7 Antibody (4E5) - BSA Free

  • c-Jun N-terminal kinase kinase 2
  • dual specificity mitogen-activated protein kinase kinase 7
  • EC 2.7.12.2
  • JNK kinase 2
  • JNK-activating kinase 2
  • JNKK 2
  • JNKK2
  • MAP kinase kinase 7
  • MAP2K7
  • MAPK/ERK kinase 7
  • MAPKK7
  • MEK 7
  • MEK7
  • mitogen-activated protein kinase kinase 7
  • MKE7
  • MKK7
  • MKK7MAPKK 7
  • PRKMK7

Background

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKK7/MEK7 Antibody (NBP2-37542) (0)

There are no publications for MKK7/MEK7 Antibody (NBP2-37542).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MKK7/MEK7 Antibody (NBP2-37542) (0)

There are no reviews for MKK7/MEK7 Antibody (NBP2-37542). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MKK7/MEK7 Antibody (NBP2-37542) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MKK7/MEK7 Products

Research Areas for MKK7/MEK7 Antibody (NBP2-37542)

Find related products by research area.

Blogs on MKK7/MEK7

There are no specific blogs for MKK7/MEK7, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MKK7/MEK7 Antibody (4E5) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol MAP2K7