MKK3/MEK3 Overexpression Lysate (Native)

Images

 
Western Blot: MEK3 Overexpression Lysate (Adult Normal) [NBL1-12848] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MEK3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MKK3/MEK3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207115 and based on accession number NM_145109. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B, mRNA.
Gene
MAP2K3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
39.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MKK3/MEK3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MKK3/MEK3 Overexpression Lysate (Native)

  • MAP kinase kinase 3
  • MAP2K3
  • MAPK/ERK kinase 3
  • MAPKK 3
  • MAPKK3PRKMK3dual specificity mitogen-activated protein kinase kinase 3
  • MEK3
  • MEK3MEK 3
  • mitogen activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 3
  • MKK3
  • MKK3EC 2.7.12.2
  • PRKMK3

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq]. Transcript Variant: This variant (B) represents the longer transcript and it encodes the longer protein (isoform B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MKK3/MEK3 Lysate (NBL1-12848) (0)

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Bioinformatics

Gene Symbol MAP2K3
Entrez