MINDY3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: FAM188A Antibody [NBP1-88543] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.

Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MINDY3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MINDY3 Antibody - BSA Free (NBP1-88543) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MSELTKELMELVWGTKSSPGLSDTIFCRWTQGFVFSESEGSALEQFEGGPCAVIAPVQAFLLKKLLFSSEKSSWRDCSEEEQKELLCHTLCDILESACCDHSGS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MINDY3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MINDY3 Recombinant Protein Antigen (NBP1-88543PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MINDY3 Antibody - BSA Free

• C10orf97
• C10orf97caspase recruitment domain containing pro-apoptotic protein
• CARPRP11-394I23.1
• chromosome 10 open reading frame 97
• Dermal papilla-derived protein 5
• DERP5CARD-containing protein
• FAM188A
• family with sequence similarity 188, member A
• FLJ13397
• MINDY3
• MST126
• MSTP126
• My042
• Protein CARP

Background

FAM188A is a member of the caspase-associated recruitment domain (CARD) family of proteins that play a role in apoptosis (Liu et al., 2002 [PubMed 12054670]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MINDY3