Human MIF ELISA Kit (Colorimetric)

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ELISA: MIF ELISA Kit [Biotin] [KA0558] - This standard curve was generated for demonstration purpose only. A standard curve must be run with each assay.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA0558
    • Availability
      Product Discontinued

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Human MIF ELISA Kit (Colorimetric) Summary

Description
MIF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human MIF.
Standard Curve Range
156 to 10000 pg/mL
Sensitivity
<20 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MIF

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes
Useful for quantification.
Publications
Read Publications using KA0558.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. 96-well plate precoated with anti-human MIF antibody
  2. ABC diluent buffer
  3. Adhesive cover
  4. Antibody diluent buffer
  5. Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  6. Biotinylated anti-human MIF antibody, dilution 1:100
  7. Lyophilized recombinant human MIF standard
  8. Sample diluent buffer
  9. TMB color developing agent
  10. TMB stop solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human MIF ELISA Kit (Colorimetric)

  • EC 5.3.2.1
  • EC 5.3.3.12
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MIF
  • MMIF
  • Phenylpyruvate tautomerase

Background

MIF encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for MIF ELISA Kit (KA0558)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MIF ELISA Kit (KA0558). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol MIF