MIF Antibody

Western Blot: MIF Antibody [NB100-1910] - Analysis of MIF expression in lysates from Jurkat (Lane 1, 2), 3T3 (Lane 3) and rat kidney (Lane 4).

Product Discontinued

MIF Antibody Summary

• Immunogen: Synthetic peptide surrounding amino acid 67 of human MIF (CAG46452).
• Specificity: MIF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MIF
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 5 - 10 ug/mL
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

May cross react with Human, pig, and bovine

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.2 mg/ml
• Purity: Affinity purified

Alternate Names for MIF Antibody

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MIF
• MMIF
• Phenylpyruvate tautomerase

Background

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibit the random migration of microphages. Recently, MIF was rediscovered as a pituitary hormone that acts as the counter-regulatory hormone for glucocorticoid action within the immune system. MIF was released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF also plays a critical role in the host control of inflammation and immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MIF
• Entrez:
  • 4282(Human)
  • 17319(Mouse)
  • 81683(Rat)
• Uniprot:
  • P14174()