MIF Antibody (2A10-4D3)

Western Blot: MIF Antibody (2A10-4D3) [H00004282-M01] - Analysis of MIF expression in transfected 293T cell line by MIF monoclonal antibody (M01), clone 2A10-4D3.Lane 1: MIF transfected lysate(12.5 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: MIF Antibody (2A10-4D3) [H00004282-M01] - Analysis of monoclonal antibody to MIF on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma tissue. Antibody concentration 1 ~ 10 ug/ml.

Immunoprecipitation: MIF Antibody (2A10-4D3) [H00004282-M01] - Analysis of MIF transfected lysate using anti-MIF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MIF MaxPab rabbit polyclonal antibody.

Sandwich ELISA: MIF Antibody (2A10-4D3) [H00004282-M01] - Detection limit for recombinant GST tagged MIF is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

MIF Antibody (2A10-4D3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MIF (AAH00447.1, 1 a.a. ~ 115 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA
• Specificity: MIF - macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MIF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MIF Antibody (2A10-4D3)

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MIF
• MMIF
• Phenylpyruvate tautomerase

Background

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIF Antibody (H00004282-M01)(2)

Publications using H00004282-M01

• Hawkins O, Verma B, Lightfoot S et al. An HLA-Presented Fragment of Macrophage Migration Inhibitory Factor Is a Therapeutic Target for Invasive Breast Cancer. J Immunol. 2011-04-22 [PMID: 21515791]
• Goh SH, Hong SH, Hong SH et al. eIF3m expression influences the regulation of tumorigenesis-related genes in human colon cancer. Oncogene. 2010-09-13 [PMID: 20838379]

Bioinformatics

• Gene Symbol: MIF
• Entrez:
  • 4282(Human)
• OMIM:
  • 153(Human)
• Uniprot:
  • AAH00447(Human)