Human Midkine ELISA Kit (Chemiluminescence)

ELISA: Human Midkine ELISA Kit (Chemiluminescence) [NBP2-66744] - Samples were spiked with high concentrations of Human Midkine and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Midkine ELISA Kit (Chemiluminescence) [NBP2-66744] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Saliva, Urine, Milk, Tissue Homogenate
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human Midkine ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human MK in samples. No significant cross-reactivity or interference between Human MK and analogues was observed.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9.97%
• Intra-Assay: CV% < 9.59%
• Spike Recovery: 90-111%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: MDK

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Midkine ELISA Kit (Chemiluminescence)

• Amphiregulin-associated protein
• ARAP
• MDK
• MEK
• Midgestation and kidney protein
• midkine (neurite growth-promoting factor 2)
• Midkine
• MK1
• MKARAP
• NEGF2
• NEGF2FLJ27379
• Neurite outgrowth-promoting factor 2
• Neurite outgrowth-promoting protein

Background

Midkine (MK) is a 13 kDa heparin-binding polypeptide which enhances neurite outgrowth, neuronal cell survival and plasminogen activator activity. MK is structurally divided into two domains, and most of the biological activities are located on the C-terminal domain. Both domains consist of three antiparallel beta-strands, but the C-terminal domain has a long flexible hairpin loop where a heparin-binding consensus sequence is located (1). MK is a member of a highly conserved, developmentally regulated human gene family. The gene product exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Expression of MK is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Midkine ELISA Kit (NBP2-66744). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MDK