Midkine Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Midkine Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant mouse Midkine
  Gly89-Asp140
  Accession # P12025
• Specificity: Detects mouse Midkine in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human Midkine is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Midkine Antibody [Alexa Fluor® 750]

• Amphiregulin-associated protein
• ARAP
• MDK
• MEK
• Midgestation and kidney protein
• midkine (neurite growth-promoting factor 2)
• Midkine
• MK1
• MKARAP
• NEGF2
• NEGF2FLJ27379
• Neurite outgrowth-promoting factor 2
• Neurite outgrowth-promoting protein

Background

Midkine (MK; also Retinoid acid-induced differentiation facor) is a secreted heparin-binding member of the very small pleiotrophin family of proteins. Although its predicted MW is 13 kDa, it runs anomalously at 15-17 kDa in SDS-PAGE. MK is strongly expressed in the embryo, but is known to be secreted in the adult by endothelium, preadipocytes, proximal renal tubular epithelium, and CD4⁺ T cells. MK has multiple activities, including the inhibition of regulatory T cell production, the promotion of adipocyte formation, and the induction of chemokine production by smooth muscle, the clustering of Ach receptors on myoblasts, and the migration of embryonic neurons plus neutrophils and macrophages. It has multiple receptors, including heparin and chondroitin sulfate, LRP-1, nucleolin, ALK, and PTP-zeta. Mature mouse midkine is 118 amino acids (aa) in length (aa 23-140). It possesses two distinct domains, an N-terminal domain spanning aa 23-71, and a C-terminal domain that encompasses aa 81-140. The C-terminal domain is further divided into two basic amino acid clusters that bind heparin. There are two splice variants reported for mouse midkine. One shows a deletion of aa 39-103, while another shows a deletion of aa 80-133. Midkine will form a covalent, crosslinked homodimer through the action of tissue type II transglutaminase. Full-length mature mouse MK (aa 23-140) shares 97% and 86% aa sequence with full-length rat and human MK, respectively..

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.