![Western Blot: MICALL1 Antibody [NBP2-70017] - Total protein from HEK293, A431 and U2OS was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MICALL1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Western-Blot-NBP2-70017-img0003.jpg "Western Blot: MICALL1 Antibody [NBP2-70017] - Total protein from HEK293, A431 and U2OS was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MICALL1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, Flow (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Synthetic peptide made to an internal portion of the human MICALL1 protein (between amino acids 200-280) [UniProt Q8N3F8] |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MICALL1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MICALL1 Antibody (NBP2-70017)Find related products by research area.
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| Gene Symbol | MICALL1 |
![Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [NBP2-70017] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MICALL1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-70017-img0001.jpg "Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [NBP2-70017] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MICALL1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: MICALL1 Antibody [NBP2-70017] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human testis using 1:200 dilution of MICALL1 antibody (NBP2-70017). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a moderate whole cell with nuclear staining.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunohistochemistry-Paraffin-NBP2-70017-img0002.jpg "Immunohistochemistry-Paraffin: MICALL1 Antibody [NBP2-70017] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human testis using 1:200 dilution of MICALL1 antibody (NBP2-70017). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a moderate whole cell with nuclear staining.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")