| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-70017SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81386-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55389-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-70017SS | |||
| BSA Free | NBP2-70017 | |||
![Western Blot: MICALL1 Antibody [H00085377-B01P] - Analysis of MICALL1 expression in transfected 293T cell line by MICALL1 polyclonal antibody. Lane 1: MICAL-L1 transfected lysate(94.93 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Western-Blot-H00085377-B01P-img0007.jpg "Western Blot: MICALL1 Antibody [H00085377-B01P] - Analysis of MICALL1 expression in transfected 293T cell line by MICALL1 polyclonal antibody. Lane 1: MICAL-L1 transfected lysate(94.93 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [H00085377-B01P] - GRAF/WDR44 label a subset of tubular endosomes. Insets show magnifications of the boxed areas. Scale bars: 10 um; scale bars of insets: 2 um. Image collected and cropped by CiteAb from the following publication (//rupress.org/jcb/article/doi/10.1083/jcb.201811014/151714/GRAF2-WDR44-and-MICAL1-mediate-Rab81011dependent) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunocytochemistry-Immunofluorescence-H00085377-B01P-img0008.jpg)
![Immunohistochemistry-Paraffin: MICALL1 Antibody [H00085377-B01P] - IHC staining of MICALL1 in human prostate cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunohistochemistry-Paraffin-H00085377-B01P-img0002.jpg)
![Western Blot: MICALL1 Antibody [H00085377-B01P] - Analysis of MICALL1 expression in HepG2.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Western-Blot-H00085377-B01P-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: MICALL1 Antibody [H00085377-B01P] - GRAF/WDR44 label a subset of tubular endosomes. (A–C) HeLa cells were incubated with methanol (vehicle) or BFA (5 µg/ml) for 5 or 15 min. (A) Percentage of cells with endogenous (endo.) WDR44 tubules. n = 7–25. (B) Confocal images of cells stained with alpha -WDR44 & alpha -TGN46 or preincubated with Alexa Fluor 546–Transferrin (10 µg/ml, 1 h). (C) Manders colocalization coefficients for the indicated proteins with endogenous WDR44 structures. n = 10–50 cells. (D) Confocal images of transfected HeLa cells showing colocalization of RFP-WDR44 with GFP-STX16 & GFP-VAMP3. (E) Immunoprecipitation (IP) of transfected 293T cells with alpha -GFP. uGRAF2 was coimmunoprecipitated by GFP-MICAL1, but not by any other member of the MICAL family. (F) Confocal images of transfected HeLa cells stained with alpha -WDR44. In the case of MICAL3pF1KA0819 & MICAL-L2, boxed areas show tubules positive only for WDR44 (red), only for MICAL3pF1KA0819/MICAL-L2 (cyan), or shared by the two proteins (white). (G) Percentage of transfected HeLa cells with endogenous (endo.) WDR44 tubules. n = 4. (H) Confocal images of untransfected HeLa cells stained with alpha -WDR44 & alpha -MICAL-L1. Boxed areas show tubules positive only for WDR44 (red), only for MICAL-L1 (cyan), or shared by the two proteins (white). (I) Internalized Integrin-beta 1 in shRNA-expressing HeLa cells after uptake of alpha -Integrin-beta 1 & following a 4-h chase. n = 4. (A, C, G, & I) Data are means ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001; & ****, P < 0.0001. (B, D, F, & H) Insets show magnifications of the boxed areas. Scale bars: 10 µm; scale bars of insets: 2 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32344433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00085377-b01p_mouse-polyclonal-micall1-antibody-31020241548220.jpg)
![Western Blot: MICALL1 Antibody [NBP2-70017] - Total protein from HEK293, A431 and U2OS was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MICALL1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Western-Blot-NBP2-70017-img0003.jpg "Western Blot: MICALL1 Antibody [NBP2-70017] - Total protein from HEK293, A431 and U2OS was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MICALL1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [NBP2-70017] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MICALL1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-70017-img0001.jpg)
![Immunohistochemistry-Paraffin: MICALL1 Antibody [NBP2-70017] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human testis using 1:200 dilution of MICALL1 antibody (NBP2-70017). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a moderate whole cell with nuclear staining.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunohistochemistry-Paraffin-NBP2-70017-img0002.jpg)
![Western Blot: MICALL1 Antibody [NBP1-81386] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/MICALL1-Antibody-Western-Blot-NBP1-81386-img0004.jpg "Western Blot: MICALL1 Antibody [NBP1-81386] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [NBP1-81386] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol, microtubule organizing center & cell junctions.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81386-img0005.jpg)
![Immunohistochemistry-Paraffin: MICALL1 Antibody [NBP1-81386] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunohistochemistry-Paraffin-NBP1-81386-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [NBP2-55389] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol, microtubule organizing center & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MICALL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55389-img0001.jpg "Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [NBP2-55389] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol, microtubule organizing center & cell junctions. Antibody staining is shown in green.")