MICAL1 Antibody (OTI6H4)

Western Blot: MICAL1 Antibody (6H4) [NBP2-03135] Analysis of extracts (35ug) from 9 different cell lines by using anti-MICAL1 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: MICAL1 Antibody (6H4) [NBP2-03135] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1.

Immunohistochemistry-Paraffin: MICAL1 Antibody (6H4) [NBP2-03135] - Staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody.

Flow Cytometry: MICAL1 Antibody (6H4) [NBP2-03135] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MICAL1 antibody, and then analyzed by flow cytometry.

Western Blot: MICAL1 Antibody (6H4) [NBP2-03135] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.

Immunohistochemistry-Paraffin: MICAL1 Antibody (6H4) [NBP2-03135] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MICAL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MICAL1 Antibody (6H4) [NBP2-03135] - Staining of paraffin-embedded Human lymph node tissue using anti-MICAL1 mouse monoclonal antibody.

Flow Cytometry: MICAL1 Antibody (6H4) [NBP2-03135] - Analysis of Jurkat cells, using anti-MICAL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: OTI6H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.93 mg/ml

MICAL1 Antibody (OTI6H4) Summary

• Immunogen: Full length human recombinant protein of human MICAL1 (NP_073602) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICAL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 117.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.93 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MICAL1 Antibody (OTI6H4)

• CasL interacting molecule
• DKFZp434B1517
• FLJ11937
• FLJ21739
• MICALMolecule interacting with CasL protein 1
• microtubule associated monoxygenase, calponin and LIM domain containing 1
• NEDD9 interacting protein with calponin homology and LIM domains
• NEDD9-interacting protein with calponin homology and LIM domains
• NICALMICAL-1

Background

MICAL1 may be a cytoskeletal regulator that connects NEDD9 to intermediate filaments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MICAL1
• Entrez:
  • 64780(Human)