MICA/B Antibody (159207) [Unconjugated]

MICA/B was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-ready
• Clone: 159207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MICA/B Antibody (159207) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human MICA
  Accession # Q29983
• Specificity: Detects human MICA/B in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICA/B Antibody (159207) [Unconjugated]

• MHC class I chain-related protein A
• MHC class I chain-related protein B
• MIC
• MICA
• MICA/B
• MICB
• PERB11
• PERB11.1
• PERB11.2

Background

MICA and MICB are stress-induced type I transmembrane proteins that function as ligands for NKG2D. They are distantly related to MHC class I proteins and are expressed on epithelial tumors and in response to infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICA/B Antibody (MAB13001)(8)

Publications using MAB13001

• CF Huang, SC Wang, WT Chang, ML Yeh, CI Huang, ZY Lin, SC Chen, WL Chuang, JF Huang, CY Dai, YL Chen, ML Yu Lower protein expression levels of MHC class I chain-related gene A in hepatocellular carcinoma are at high risk of recurrence after surgical resection Sci Rep, 2018;8(1):15821. 2018 [PMID: 30361527] (IHC, Human)
• Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity. Eur J Immunol, 2015;45(8):2324-34. 2015 [PMID: 25991034] (Immunoprecipitation, Human)
• Kim H, Bose S, Meyer K, Ray R Hepatitis C virus impairs natural killer cell-mediated augmentation of complement synthesis. J Virol, 2014;88(5):2564-71. 2014 [PMID: 24352446] (Western Blot, Human)
• Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. PLoS Pathog., 2012;8(1):e1002486. 2012 [PMID: 22253598] (Flow Cytometry, Human)
• Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells. PLoS ONE, 2011;6(2):e16899. 2011 [PMID: 21364924] (Western Blot, Human)
• Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma. Oncogene, 2011;30(23):2622-32. 2011 [PMID: 21258414] (Flow Cytometry, Human)
• Roda-Navarro P, Vales-Gomez M, Chisholm SE, Reyburn HT Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function. Proc. Natl. Acad. Sci. U.S.A., 2006;103(30):11258-63. 2006 [PMID: 16849432] (ICC, Human)
• Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B. Cancer Res., 2005;65(23):11136-45. 2005 [PMID: 16322264] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: MICA
• Uniprot:
  • Q29983()