MICA/B Antibody (159207) [Phycoerythrin]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B PE-conjugated Monoclonal Antibody (Catalog # FAB13001P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 159207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

MICA/B Antibody (159207) [Phycoerythrin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human MICA
  Accession # Q29983
• Specificity: Detects human MICA/B in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICA/B Antibody (159207) [Phycoerythrin]

• MHC class I chain-related protein A
• MHC class I chain-related protein B
• MIC
• MICA
• MICA/B
• MICB
• PERB11
• PERB11.1
• PERB11.2

Background

MICA and MICB are stress-induced type I transmembrane proteins that function as ligands for NKG2D. They are distantly related to MHC class I proteins and are expressed on epithelial tumors and in response to infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICA/B Antibody (FAB13001P)(8)

Publications using FAB13001P

• Lee, S;Chae, SJ;Jang, IH;Oh, SC;Kim, SM;Lee, SY;Kim, JH;Ko, J;Kim, HJ;Song, IC;Kim, JK;Kim, TD; B7H6 is the predominant activating ligand driving natural killer cell-mediated killing in patients with liquid tumours: evidence from clinical, in silico, in vitro, and in vivo studies EBioMedicine 2024-11-22 [PMID: 39579618] (FACS, Human)
• Q Duan, H Li, C Gao, H Zhao, S Wu, H Wu, C Wang, Q Shen, T Yin High glucose promotes pancreatic cancer cells to escape from immune surveillance via AMPK-Bmi1-GATA2-MICA/B pathway J. Exp. Clin. Cancer Res., 2019-05-14;38(1):192. 2019-05-14 [PMID: 31088566] (Flow Cytometry, Human)
• JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells Oncotarget, 2017-01-01;0(0):. 2017-01-01 [PMID: 28615524] (Flow Cytometry, Human)
• Morisaki T, Hirano T, Koya N, Kiyota A, Tanaka H, Umebayashi M, Onishi H, Katano M NKG2D-directed cytokine-activated killer lymphocyte therapy combined with gemcitabine for patients with chemoresistant metastatic solid tumors. Anticancer Res, 2014-08-01;34(8):4529-38. 2014-08-01 [PMID: 25075096] (Flow Cytometry, Human)
• Umebayashi M, Kiyota A, Koya N, Tanaka H, Onishi H, Katano M, Morisaki T An epithelial cell adhesion molecule- and CD3-bispecific antibody plus activated T-cells can eradicate chemoresistant cancer stem-like pancreatic carcinoma cells in vitro. Anticancer Res, 2014-08-01;34(8):4509-19. 2014-08-01 [PMID: 25075094] (Flow Cytometry, Human)
• Yamanegi K, Yamane J, Kobayashi K Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma cells to Fas- and NK cell-mediated cell death. Int. J. Oncol., 2012-04-19;41(1):83-91. 2012-04-19 [PMID: 22576685] (Flow Cytometry, Human)
• Morisaki T, Onishi H, Koya N Combinatorial Cytotoxicity of Gemcitabine and Cytokine-activated Killer Cells in Hepatocellular Carcinoma via the NKG2D-MICA/B System. Anticancer Res., 2011-07-01;31(7):2505-10. 2011-07-01 [PMID: 21873167] (Flow Cytometry, Human)
• Qin H, Lee IF, Panagiotopoulos C, Wang X, Chu AD, Utz PJ, Priatel JJ, Tan R Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling. Diabetes, 2011-01-26;60(3):857-66. 2011-01-26 [PMID: 21270236] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: MICA
• Uniprot:
  • Q29983()