MICA/B Antibody (159207) [DyLight 650]

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-ready
• Clone: 159207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

MICA/B Antibody (159207) [DyLight 650] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human MICA
  Accession # Q29983
• Specificity: Detects human MICA/B in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MICA/B Antibody (159207) [DyLight 650]

• MHC class I chain-related protein A
• MHC class I chain-related protein B
• MIC
• MICA
• MICA/B
• MICB
• PERB11
• PERB11.1
• PERB11.2

Background

The MIC gene is located in MHC class I region. MICA/B are 65-75 kD stress-inducible glycoproteins with highly polymorphic. They are MHC class I-like transmembrane molecules that do not associate beta2-microglobulin and do not present peptides. MICA and MICB share 85% identify, and are mainly expressed on Intestinal epithelial cells, epithelial tumor cells, endothelial cells, fibroblasts, and IFN- alpha-stimulated dendritic cells. MIC molecules bind NKG2D, an activating receptor, and induce activation of NK cells, and subset of CD8+ alpha/ beta T cells and gamma/ delta T cells, as well as suppression of T cell proliferation. MICA/B recognition is involved in the regulation of tumor surveillance, viral infection and autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MICA