MICA Antibody - BSA Free

Images

 
Western Blot: MICA Antibody [NBP1-76805] - A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: MICA Antibody [NBP1-76805] - analysis of MICA in Jukat cells using anti-MICA antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: MICA Antibody [NBP1-76805] - Human colon tissue with MICA antibody at 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: MICA Antibody [NBP1-76805] - in Human Colon cells with MICA

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price

MICA Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA. The immunogen is located within the last 50 amino acids of MICA. Amino Acid Squence: QHPVGTSDHRDATQLG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MICA
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml
Application Notes
MICA antibody validated for ICC/IF from a verified customer review.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
MICA Peptide (NBP1-76805PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-76805 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for MICA Antibody - BSA Free

  • FLJ60820
  • MGC111087
  • MICA
  • PERB11.1

Background

Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MICA Antibody (NBP1-76805) (0)

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Review for MICA Antibody (NBP1-76805) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-76805:
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Images Ratings Applications Species Date Details
Immunofluorescence MICA NBP1-76805
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4
reviewed by:
Verified Customer
IF Human 08/07/2015
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Summary

ApplicationImmunofluorescence
Sample TestedCell culture
SpeciesHuman

Blocking

Blocking DetailsBlocked using animal-free blocking reagent

Secondary Antibody

Secondary DescriptionGoat anti-rabbit DyLight 594 secondary antibody

Details

Detection NotesDetected using fluorescent microscopy
Fixation DetailsCytospin fixed with 90% MeOH at -20 degrees C, no antigen retrieval required.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MICA Antibody (NBP1-76805) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MICA Antibody (NBP1-76805)

Discover related pathways, diseases and genes to MICA Antibody (NBP1-76805). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MICA Antibody (NBP1-76805)

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Pathways for MICA Antibody (NBP1-76805)

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Blogs on MICA.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

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Recent Reviews

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Verified Customer
08/07/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol MICA
Uniprot