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MICA Antibody - BSA Free

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Western Blot: MICA Antibody [NBP1-76805] - A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Western Blot: MICA Antibody [NBP1-76805] - A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: MICA Antibody [NBP1-76805] - analysis of MICA in Jukat cells using anti-MICA antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: MICA Antibody [NBP1-76805] - Human colon tissue with MICA antibody at 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: MICA Antibody [NBP1-76805] - in Human Colon cells with MICA

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-76805-0.025mg
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MICA Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA. The immunogen is located within the last 50 amino acids of MICA. Amino Acid Squence: QHPVGTSDHRDATQLG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MICA
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml
Application Notes
MICA antibody validated for ICC/IF from a verified customer review.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
MICA Peptide (NBP1-76805PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-76805 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for MICA Antibody - BSA Free

  • FLJ60820
  • MGC111087
  • MICA
  • PERB11.1

Background

Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of MCF-7 cells using HLA-B mouse mAb (green) and negative control (red).
Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Flow Cytometry: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SEC14L2 antibody, and then analyzed by flow cytometry.
Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
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(384 Publications)
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HLA G Antibody
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Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on Hek293 cells with HLA G Antibody [87G] NBP1-43123AF488 (blue) and a matched isotype control (orange). Both antibodies were conjugated to Alexa Fluor 488. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature.
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Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.
Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.
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Immunohistochemistry-Paraffin: NXF1 Antibody [NBP1-31649] - Paraffin-embedded NCIN87 Xenograft, using antibody at 1:100 dilution.
Western Blot: NXF1 Antibody [NBP1-31649] - Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: NXF1 Antibody [NBP1-31649] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
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Publications for MICA Antibody (NBP1-76805) (0)

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Review for MICA Antibody (NBP1-76805) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-76805:
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Immunofluorescence MICA NBP1-76805
Enlarge 		4
reviewed by:
Verified Customer
 IF Human 08/07/2015
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Summary

ApplicationImmunofluorescence
Sample TestedCell culture
SpeciesHuman

Blocking

Blocking DetailsBlocked using animal-free blocking reagent

Secondary Antibody

Secondary DescriptionGoat anti-rabbit DyLight 594 secondary antibody

Details

Detection NotesDetected using fluorescent microscopy
Fixation DetailsCytospin fixed with 90% MeOH at -20 degrees C, no antigen retrieval required.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MICA Antibody (NBP1-76805) (0)

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Blogs on MICA.

Check out the latest blog posts on MICA.
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Immunofluorescence MICA NBP1-76805
Enlarge
 Verified Customer
08/07/2015
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol MICA
Uniprot