MICA Antibody - BSA Free

Western Blot: MICA Antibody [NBP1-76805] - A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Immunocytochemistry/Immunofluorescence: MICA Antibody [NBP1-76805] - analysis of MICA in Jukat cells using anti-MICA antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: MICA Antibody [NBP1-76805] - Human colon tissue with MICA antibody at 10 ug/ml.

Immunocytochemistry/ Immunofluorescence: MICA Antibody - BSA Free [NBP1-76805] - Immunofluorescence of MICA in Human Colon tissue with MICA antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MICA Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MICA Antibody - BSA Free (NBP1-76805) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA. The immunogen is located within the last 50 amino acids of MICA. Amino Acid Squence: QHPVGTSDHRDATQLG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MICA
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: MICA antibody validated for ICC/IF from a verified customer review.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  MICA Peptide (NBP1-76805PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MICA Antibody - BSA Free

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MICA Antibody (NBP1-76805) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 08/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Cell culture
• Species: Human

Bioinformatics

• Gene Symbol: MICA
• Uniprot:
  • NP_000238()