MICA Antibody (159227) [Unconjugated]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

MICA knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the MICA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of n≥3) for surface expression of ligands on activated (thick black line) and resting CD4+ T cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated CD4+ T) and filled histogram (resting CD4+ T). Middle- and right column: NK and CD4+ T cells were activated for 4 days in vitro as described, and co-cultured for 4 hours with 10 ug/mL mAb (or relevant isotype-matched control Ig). Degranulation is shown for CD56dim (middle column) and CD56bright (right column) NK cells. Representative histograms of surface expression of receptors on activated (thick black line) and resting NK cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated NK) and filled histogram (resting NK). * P<0.05, ** P<0.005, *** P<0.001. (C) Sorted IL-2-activated CD56dim and CD56bright NK cells were co-cultured with 51Cr-labeled activated CD4+ T cells in a 51Cr-release assay with human IgG4 isotype control (•) or anti-NKG2A mAb (○). Data represents n = 3 experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22384114), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready, KO
• Clone: 159227
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MICA Antibody (159227) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human MICA
• Specificity: Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MICB is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICA Antibody (159227) [Unconjugated]

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. NKG2D and its Ligands (2002) http://www.RnDSystems.com

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICA Antibody (MAB1300)(40)

Publications using MAB1300

• Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS; Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters 2023-06-01 [PMID: 37153058] (Flow Cytometry, Human)
• J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022-03-25;13(1):1606. 2022-03-25 [PMID: 35338135] (Western Blot, Human)
• S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021-09-04;12(9):836. 2021-09-04 [PMID: 34482362] (Flow Cytometry, Human)
• E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021-05-03;17(5):e1008807. 2021-05-03 [PMID: 33939764] (Flow Cytometry, ICC, Human)
• E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain Nature Communications, 2021-04-06;12(1):2061. 2021-04-06 [PMID: 33824318] (Flow Cytometry, ICC, Human)
• Adeline Raynaud, Klervi Desrumeaux, Laurent Vidard, Elise Termine, Daniel Baty, Patrick Chames et al. Anti-NKG2D single domain-based antibodies for the modulation of anti-tumor immune response OncoImmunology 1/18/2021 [PMID: 33457075]
• M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020-07-20;12(7):. 2020-07-20 [PMID: 32698530] (Flow Cytometry, Human)
• SH Chang, HJ Kim, CG Park Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens Cells, 2020-05-18;9(5):. 2020-05-18 [PMID: 32443511] (Flow Cytometry, Human)
• M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production Cell. Mol. Immunol., 2019-08-30;0(0):. 2019-08-30 [PMID: 31471588] (Flow Cytometry, Human)
• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019-06-11;5(0):. 2019-06-11 [PMID: 31184599] (IHC, Human)
• MJ Sakiyama, I Espinoza, A Reddy, F de Carlo, A Kumar, AS Levenson, S Bae, X Zhou, PP Claudio, J Lewin, V Manucha, CR Pound, S Vijayakuma, CR Gomez Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancer Exp. Mol. Pathol., 2019-04-17;0(0):. 2019-04-17 [PMID: 31004600] (Flow Cytometry, Human)
• MP Abruzzese, MT Bilotta, C Fionda, A Zingoni, A Soriani, MT Petrucci, MR Ricciardi, R Molfetta, R Paolini, A Santoni, M Cippitelli The homeobox transcription factor MEIS2 is a regulator of cancer cell survival and IMiDs activity in Multiple Myeloma: modulation by Bromodomain and Extra-Terminal (BET) protein inhibitors Cell Death Dis, 2019-04-11;10(4):324. 2019-04-11 [PMID: 30975979] (Flow Cytometry, Human)
• Da Lin, Thomas K Hiron, Christopher A O'Callaghan Intragenic transcriptional interference regulates the human immune ligand MICA The EMBO Journal 2018-05-15 [PMID: 29643123] (Flow Cytometry, Human)
• Da Lin, Thomas K Hiron, Christopher A O'Callaghan Intragenic transcriptional interference regulates the human immune ligand MICA The EMBO Journal 5/15/2018 [PMID: 29643123]
• Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20 Sci Rep, 2017-08-17;7(1):8661. 2017-08-17 [PMID: 28819195] (Flow Cytometry, Human)
• C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells Cell Death Dis, 2017-08-03;8(8):e2973. 2017-08-03 [PMID: 28771222] (Flow Cytometry, Human)
• AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 2017-05-09;8(19):31959-31976. 2017-05-09 [PMID: 28404876] (Flow Cytometry, Human)
• O Berhani, D Nachmani, R Yamin, D Schmiedel, Y Bar-On, O Mandelboim Vigilin Regulates the Expression of the Stress-Induced Ligand MICB by Interacting with Its 5&#039; Untranslated Region J. Immunol., 2017-03-29;0(0):. 2017-03-29 [PMID: 28356383] (Flow Cytometry, Human)
• M Diab, A Glasner, B Issacson, Y Bar-On, Y Drori, R Yamin, A Duev-Cohen, O Danziger, R Zamostiano, M Mandelboim, S Jonjic, E Bacharach, O Mandelboim NK-cell receptors NKp46 and NCR1 control human metapneumovirus infection Eur. J. Immunol, 2017-03-06;0(0):. 2017-03-06 [PMID: 28191644] (Flow Cytometry, Human)
• Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study Sci Rep, 2016-12-02;6(0):38407. 2016-12-02 [PMID: 27910927] (Neutralization, Human)
• Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells Sci Rep, 2016-09-27;6(0):32470. 2016-09-27 [PMID: 27671170] (Flow Cytometry, Human)
• Forslund E, Sohlberg E, Enqvist M, Olofsson P, Malmberg K, Onfelt B Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells. J Immunol, 2015-08-28;195(7):3374-81. 2015-08-28 [PMID: 26320254] (Flow Cytometry, Human)
• Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol, 2015-06-12;195(2):736-48. 2015-06-12 [PMID: 26071561] (Neutralization, Human)
• Enqvist M, Ask E, Forslund E, Carlsten M, Abrahamsen G, Beziat V, Andersson S, Schaffer M, Spurkland A, Bryceson Y, Onfelt B, Malmberg K Coordinated expression of DNAM-1 and LFA-1 in educated NK cells. J Immunol, 2015-03-30;194(9):4518-27. 2015-03-30 [PMID: 25825444] (Flow Cytometry, Human)
• Kim , Ga Rim, Ha , Ga-Hee, Bae , Jae-Ho, Oh , Sae-Ock, Kim , Sun-Hee, Kang , Chi-Dug Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells. Oncol Lett, 2015-01-29;9(4):1641-1646. 2015-01-29 [PMID: 25789015] (Flow Cytometry, Human)
• Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 2015-01-29;135(5):1311-9. 2015-01-29 [PMID: 25634359] (FACS, Human)
• Nachmani , Daphna, Gutschner , Tony, Reches , Adi, Diederichs , Sven, Mandelboim , Ofer RNA-binding proteins regulate the expression of the immune activating ligand MICB. Nat Commun, 2014-06-13;5(0):4186. 2014-06-13 [PMID: 24924487] (Flow Cytometry, Human)
• Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1. J Immunol, 2014-06-09;193(2):950-60. 2014-06-09 [PMID: 24913980] (Flow Cytometry, Human)
• Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014-04-30;192(11):5332-42. 2014-04-30 [PMID: 24790151] (Neutralization, Human)
• Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, Davies J, McSharry B, Weekes M, Antrobus P, Prod'homme V, Blanchet F, Sugrue D, Cuff S, Roberts D, Davison A, Lehner P, Wilkinson G, Tomasec P Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. PLoS Pathog, 2014-05-01;10(5):e1004058. 2014-05-01 [PMID: 24787765] (Neutralization, Human)
• Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O MicroRNA editing facilitates immune elimination of HCMV infected cells. PLoS Pathog, 2014-02-27;10(2):e1003963. 2014-02-27 [PMID: 24586166] (Flow Cytometry, Human)
• Annabelle Chauveau, Pierre Tonnerre, Angélique Pabois, Pierre-Jean Gavlovsky, Mathais Chatelais, Stéphanie Coupel et al. Endothelial Cell Activation and Proliferation Modulate NKG2D Activity by Regulating MICA Expression and Shedding Journal of Innate Immunity 1/1/2014 [PMID: 23860405]
• Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells. Cancer Res, 2012-08-21;72(21):5463-72. 2012-08-21 [PMID: 22915757] (Flow Cytometry, Human)
• Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Lab. Invest., 2012-03-26;92(7):967-77. 2012-03-26 [PMID: 22449797] (Flow Cytometry, Human)
• Nielsen N, Odum N, Urso B, Lanier LL, Spee P Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE, 2012-02-22;7(2):e31959. 2012-02-22 [PMID: 22384114] (Flow Cytometry, Human)
• Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells. PLoS ONE, 2011-02-25;6(2):e16899. 2011-02-25 [PMID: 21364924] (Flow Cytometry, Western Blot, Human)
• Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma. Oncogene, 2011-01-24;30(23):2622-32. 2011-01-24 [PMID: 21258414] (Flow Cytometry, Human)
• Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J. Exp. Med., 2010-12-20;208(1):13-21. 2010-12-20 [PMID: 21173105] (Flow Cytometry, Human)
• Cerboni C, Ardolino M, Santoni A, Zingoni A Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood, 2009-01-05;113(13):2955-64. 2009-01-05 [PMID: 19124832] (Neutralization, Human)
• Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol. Immunol., 2008-07-26;45(15):3917-25. 2008-07-26 [PMID: 18657862] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: MICA