MICA Antibody (159227) [Phycoerythrin]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE-conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

MICA knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE-conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the MICA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Expression of the Sudan virus (SUDV)- or Makona-GP results in loss of surface staining of alpha MICA in HEK293T cells and JEG3 cells stably transfected with MICA. The figure shows representative flow cytometry analysis for steric shielding of the MICA antigen by SUDV-GP or Makona-GP. (A) HEK293T cells were transfected, harvested, and stained for SUDV-GP with a biotinylated 3C10 antibody, followed by allophycocyanin (APC)-conjugated streptavidin, and co-stained for MICA surface antigens with phycoerythrin (PE)-conjugated alpha MICA antibody. Results are from one representative experiment of more than 10 performed (B) HEK293T cells were transfected, harvested, and stained for Makona-GP with survivor sera, followed by APC-labeled secondary antibody, and co-stained for MICA surface antigen using PE-conjugated alpha MICA antibody. Results are from one representative experiment of two performed. (C) SUDV-GP-transfected JEG3-MICA cells stained for SUDV-GP and co-stained for MICA surface antigens and NKG2D-Ig ligands. Results are from one representative experiment of more than four performed. (D–F) HEK293T cells were transfected with SUDV-GP or SUDV-GP-GFP, harvested shortly after they were treated with trypsin or left untreated, and finally stained for: (D) HLA-A, B, C, and SUDV-GP, (E) MICA and SUDV-GP, and (F) B7H6. Results are from one representative experiment of more than four performed. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30013549), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, KO
• Clone: 159227
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

MICA Antibody (159227) [Phycoerythrin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human MICA
• Specificity: Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human MICB.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells
  • Knockout Validated 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICA Antibody (159227) [Phycoerythrin]

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. NKG2D and its Ligands (2002) http://www.RnDSystems.com

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICA Antibody (FAB1300P)(18)

Publications using FAB1300P

• Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS; Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters 2023-06-01 [PMID: 37153058] (Flow Cytometry, Human)
• Chiao-Ling Tsai, Po-Sheng Yang, Feng-Ming Hsu, Ann-Lii Cheng, Wan-Ni Yu, Jason Chia-Hsien Cheng Topoisomerase I Inhibition Radiosensitizing Hepatocellular Carcinoma by RNF144A-mediated DNA-PKcs Ubiquitination and Natural Killer Cell Cytotoxicity Journal of Clinical and Translational Hepatology 2023-06-28 [PMID: 36969901]
• DR Calabrese, T Tsao, M Magnen, C Valet, Y Gao, B Mallavia, JJ Tian, EA Aminian, KM Wang, A Shemesh, EB Punzalan, A Sarma, CS Calfee, SA Christenso, CR Langelier, SR Hays, JA Golden, LE Leard, ME Kleinhenz, NA Kolaitis, RJ Shah, A Venado, LL Lanier, JR Greenland, DM Sayah, A Ardehali, J Kukreja, SS Weigt, JA Belperio, JP Singer, MR Looney NKG2D receptor activation drives primary graft dysfunction severity and poor lung transplantation outcomes JCI Insight, 2022-12-22;0(0):. 2022-12-22 [PMID: 36346670] (Flow Cytometry, Human)
• SS Dinavahi, YC Chen, K Punnath, A Berg, M Herlyn, M Foroutan, ND Huntington, GP Robertson Targeting WEE1/AKT restores p53-dependent NK cell activation to induce immune checkpoint blockade responses in 'cold' melanoma Cancer Immunology Research, 2022-06-03;0(0):. 2022-06-03 [PMID: 35439317] (FACS, Mouse)
• S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021-09-04;12(9):836. 2021-09-04 [PMID: 34482362] (Flow Cytometry, Human)
• JH Jeon, IC Baek, CH Hong, KH Park, H Lee, EJ Oh, TG Kim Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies Scientific Reports, 2021-08-03;11(1):15716. 2021-08-03 [PMID: 34344955] (Flow Cytometry, Human)
• Y Zhu, J Xie, J Shi Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis Bmc Biology, 2021-07-30;19(1):140. 2021-07-30 [PMID: 34325694] (Flow Cytometry, Human)
• S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors Oncoimmunology, 2017-11-06;7(2):e1392426. 2017-11-06 [PMID: 29308322] (Flow Cytometry, Human)
• XY Fan, PY Wang, C Zhang, YL Zhang, Y Fu, C Zhang, QX Li, JN Zhou, BE Shan, DW He All-trans retinoic acid enhances cytotoxicity of CIK cells against human lung adenocarcinoma by upregulating MICA and IL-2 secretion Sci Rep, 2017-11-28;7(1):16481. 2017-11-28 [PMID: 29184163] (Flow Cytometry, Human)
• Jie Pan, Jiaying Shen, Wengong Si, Chengyong Du, Danni Chen, Liang Xu et al. Resveratrol promotes MICA/B expression and natural killer cell lysis of breast cancer cells by suppressing c-Myc/miR-17 pathway Oncotarget 9/12/2017 [PMID: 29029468]
• Amanda R. Campbell, Megan C. Duggan, Lorena P. Suarez-Kelly, Neela Bhave, Kallan S. Opheim, Elizabeth L. McMichael et al. MICA-Expressing Monocytes Enhance Natural Killer Cell Fc Receptor-Mediated Antitumor Functions Cancer Immunology Research 2017-09-01 [PMID: 28724544]
• C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma Oncotarget, 2017-01-24;8(4):5954-5964. 2017-01-24 [PMID: 27992381] (Flow Cytometry, Human)
• M Ke, H Wang, Y Zhou, J Li, Y Liu, M Zhang, J Dou, T Xi, B Shen, C Zhou SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice Oncotarget, 2016-08-02;7(31):49509-49526. 2016-08-02 [PMID: 27385218] (Flow Cytometry, Human)
• Gomez-Lomeli P, Bravo-Cuellar A, Hernandez-Flores G, Jave-Suarez L, Aguilar-Lemarroy A, Lerma-Diaz J, Dominguez-Rodriguez J, Sanchez-Reyes K, Ortiz-Lazareno P Increase of IFN-gamma and TNF-alpha production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor. Cancer Cell Int, 2014-10-01;14(1):100. 2014-10-01 [PMID: 25302050] (Flow Cytometry, Human)
• Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014-04-30;192(11):5332-42. 2014-04-30 [PMID: 24790151] (Flow Cytometry, Human)
• Okita R, Mougiakakos D, Ando T, Mao Y, Sarhan D, Wennerberg E, Seliger B, Lundqvist A, Mimura K, Kiessling R HER2/HER3 signaling regulates NK cell-mediated cytotoxicity via MHC class I chain-related molecule A and B expression in human breast cancer cell lines. J. Immunol., 2012-02-01;188(5):2136-45. 2012-02-01 [PMID: 22301547] (Flow Cytometry, Human)
• Lanca T, Correia DV, Moita CF, Raquel H, Neves-Costa A, Ferreira C, Ramalho JS, Barata JT, Moita LF, Gomes AQ, Silva-Santos B The MHC class Ib protein ULBP1 is a nonredundant determinant of leukemia/lymphoma susceptibility to gammadelta T-cell cytotoxicity. Blood, 2010-01-25;115(12):2407-11. 2010-01-25 [PMID: 20101024] (Flow Cytometry, Human)
• Dibra D, Cutrera JJ, Xia X, Birkenbach MP, Li S Expression of WSX1 in tumors sensitizes IL-27 signaling-independent natural killer cell surveillance. Cancer Res., 2009-06-23;69(13):5505-13. 2009-06-23 [PMID: 19549909] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: MICA