MICA Antibody (159227) [Alexa Fluor® 488]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1300G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 159227
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

MICA Antibody (159227) [Alexa Fluor® 488] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human MICA
• Specificity: Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human MICB.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICA Antibody (159227) [Alexa Fluor® 488]

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. NKG2D and its Ligands (2002) http://www.RnDSystems.com

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICA Antibody (FAB1300G)(5)

Publications using FAB1300G

• Morimoto Y, Yamashita N, Daimon T et al. MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells Journal for immunotherapy of cancer 2023-02-01 [PMID: 36754452]
• R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (ICC, Human)
• K Goto, J Arai, A Stephanou, N Kato Novel therapeutic features of disulfiram against hepatocellular carcinoma cells with inhibitory effects on a disintegrin and metalloproteinase 10 Oncotarget, 2018-04-10;9(27):18821-18831. 2018-04-10 [PMID: 29721164] (Flow Cytometry, Human)
• Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study Sci Rep, 2016-12-02;6(0):38407. 2016-12-02 [PMID: 27910927] (Flow Cytometry, Human)
• Samanta R Zanetti Expression and Functional Role of ?7 Nicotinic Receptor in Human Cytokine-Stimulated NK Cells J Biol Chem, 2016-06-09;0(0):. 2016-06-09 [PMID: 27284006] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: MICA