mGluR8 Overexpression Lysate (Native)

Images

 
Western Blot: metabotropic Glutamate Receptor 8 Overexpression Lysate (Adult Normal) [NBL1-11352] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for metabotropic ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

mGluR8 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210813 and based on accession number NM_000845. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens glutamate receptor, metabotropic 8 (GRM8), transcript variant 1, mRNA.
Gene
GRM8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
97.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for mGluR8

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for mGluR8 Overexpression Lysate (Native)

  • FLJ41058
  • glutamate metabotropic receptor 8
  • glutamate receptor, metabotropic 8
  • GPRC1H
  • GPRC1HMGLUR8GLUR8
  • GRM8
  • metabotropic glutamate receptor 8
  • MGC126724
  • mGlu8
  • mGluR8

Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for mGluR8 Lysate (NBL1-11352) (0)

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Bioinformatics

Gene Symbol GRM8
Entrez