mGluR7 Antibody - BSA Free

Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS917] - Analysis of anti-GRM7 / MGLUR7 antibody with human adrenal at dilution 7 ug/ml.

Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS917] - Analysis of anti-GRM7 / MGLUR7 antibody with human liver at dilution 7 ug/ml.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Eq, Pm, Pm, Pm, Pm-Or
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

mGluR7 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human mGluR7.
• Epitope: N-Terminus extracellular domain
• Localization: Cell membrane; Multi-pass membrane protein.
• Specificity: Human mGluR7. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRM4 (60%), GRM6 (45%).
• Predicted Species: Orangutan (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 7 ug/ml
  • Western Blot 1:1000 - 1:10000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 24858010)..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Opossum (80%), Turkey (80%), Chicken (80%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for mGluR7 Antibody - BSA Free

• FLJ40498
• glutamate receptor, metabotropic 7
• GPRC1G
• GRM7
• metabotropic glutamate receptor 7
• MGLU7
• mGluR7
• MGLUR7GPRC1GGLUR7

Background

Metabotropic Glutamate Receptor GRM7 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. GRM7 is highly concentrated at presynaptic terminals and is thought to play an important role in the modulation of glutamatergic synaptic transmission by effecting presynaptic inhibition of glutamate release. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group III includes GRM4, GRM6, GRM7, and GRM8 receptors, which are linked to inhibition of the cyclic AMP signaling cascade. Five mRNA variants of GRM7 have been described; most of the variants are expressed in brain and retina, although there are non-neuronal variants that may have different functional properties. GRM7 has been reported to be expressed in active zones of presynaptic axon terminals in various regions of the brain, eye, and spinal cord. Caution: GLUR7 refers to Ionotropic Glutamate Receptor 7, not to Metabotropic Glutamate Receptor 7 (mGLUR7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mGluR7 Antibody (NLS917)(2)

Publications using NLS917

• Fujikawa T, Petralia RS, Fitzgerald TS et al. Localization of kainate receptors in inner and outer hair cell synapses. Hear. Res. 2014-05-21 [PMID: 24858010] (ICC/IF, Rat)
  
  Details:
  Rat cochlear tissue, Fig S6. Cochlear tissue was fixed in 4% paraformaldehyde for 1 h at 4 degrees C and permeabilized with 0.5% Triton X-100 prior to incubation with the Metabotropic Glutamate Receptor pAb.
• Friedman RA, Van Laer L, Huentelman MJ et al. GRM7 variants confer susceptibility to age-related hearing impairment. Hum Mol Genet. 2009-02-15 [PMID: 19047183] (IHC-P, Human)
  
  Details:
  IHC (paraffin), mouse ear tissue, Fig. 3A,B,C,F; IHC (paraffin), human temporal bone tissue, Fig. 4.

Bioinformatics

• Gene Symbol: GRM7
• Entrez:
  • 2917(Human)