Bassoon Antibody (SAP7F407)


Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.
Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.

Product Details

Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

Order Details

Bassoon Antibody (SAP7F407) Summary

Recombinant rat Bassoon.
This affinity purified detects an ~400 kDa protein, corresponding to the apparent molecular mass of Bassoon on SDSPAGE immunoblots, in samples from mouse and rat origins. Additional bands between 97 and 400 kDa may also be detected.
Protein G purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Liquid. In PBS, pH 7.2, containing 50% glycerol
0.09% Sodium Azide
1.0 mg/ml
Protein G purified


Application Notes
WB: Detects a band of approximately 400 kDa (predicted molecular weight: 472.6 kDa). Additional bands between 97 and 400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon. Optimal dilutions/concentrations should be determined by the end user. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
416 kDa
Read Publication using
NB120-13249 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Bassoon Antibody (SAP7F407)

  • bassoon (presynaptic cytomatrix protein)


Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bassoon Antibody (NB120-13249)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC/IF.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bassoon Antibody (NB120-13249). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?
    • After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

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Isotype Controls

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