Bassoon Antibody (SAP7F407)

Images

 
Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.
Immunocytochemistry/ Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat Hippocampal Neuron, image courtesy of customer.
Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
SAP7F407
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Bassoon Antibody (SAP7F407) Summary

Immunogen
Recombinant rat Bassoon.
Specificity
This affinity purified detects an ~400 kDa protein, corresponding to the apparent molecular mass of Bassoon on SDSPAGE immunoblots, in samples from mouse and rat origins. Additional bands between 97 and 400 kDa may also be detected.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BSN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2.5 ug
Application Notes
Additional bands between 97 and 400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon. Optimal dilutions/concentrations should be determined by the end user. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28821669).
Theoretical MW
400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB120-13249 in the following applications:

Publications
Read Publications using
NB120-13249 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Bassoon Antibody (SAP7F407)

  • bassoon (presynaptic cytomatrix protein)

Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bassoon Antibody (NB120-13249)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: ICC/IF, IHC-FR.


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ICC/IF
(1)
IHC-FR
(1)
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Rat
(1)
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Review for Bassoon Antibody (NB120-13249) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: ICC.

Reviews using NB120-13249:
Filter by Applications
ICC
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Bassoon NB120-13249
Enlarge
reviewed by:
Janosch Heller
ICC Rat 03/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured rat hippocampal neurons
SpeciesRat

Comments

CommentsFixation: 3% formaldehyde (TAAB) in PBS, 15 minutes RT
Blocking: 2% milk powder in PBS, 1 hour RT
Permeabilization: 0.2% Triton-X100
Primary Antibody: 1:100 in 1% milk powder in PBS with 0.2% Triton, overnight, 4°C
Wash: three times wash with PBS with 0.2% Triton, 10 min each
Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:500 in PBS with 0.2% Triton, 2 hours, RT
Wash: three times wash with PBS, 10 min each
Microscope: Confocal (Zeiss LSM710)

The antibody labels clusters along the dendrites and somata of the cultured cells as expected.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bassoon Antibody (NB120-13249). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?
    • After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Janosch Heller
03/27/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol BSN
Entrez
Uniprot