MFG-E8 Knockout 293T Cell Lysate Summary
Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 2
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.
Packaging, Storage & Formulations
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.
Lysate Details for Array
Knockout 293T Cell
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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.
Alternate Names for MFG-E8 Knockout 293T Cell Lysate
- Breast epithelial antigen BA46
- milk fat globule-EGF factor 8 protein
- Milk fat globule-EGF factor 8
- O-acetyl disialoganglioside synthase
- sperm associated antigen 10
- sperm surface protein hP47
This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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