Mouse MFG-E8 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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    • Catalog Number
      KA1802
    • Availability
      Product Discontinued

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Mouse MFG-E8 ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve. Mfge8 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Mfge8.
Sensitivity
1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MFGE8

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Detection Antibody MFG-E8 (Item F): 2 vial of biotinylated anti-mouse MFG-E8 (each vial is enough to assay half microplate).
  2. HRP-Streptavidin Concentrate (Item G): 8 µl 20,000x concentrated HRP-conjugated streptavidin.
  3. MFG-E8 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse MFG-E8
  4. Standards (Item C): 2 vials of recombinant mouse MFG-E8.
  5. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  6. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  7. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse MFG-E8 ELISA Kit (Colorimetric)

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47

Background

Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for MFG-E8 ELISA Kit (KA1802). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol MFGE8