Mouse MFG-E8 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. Mfge8 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Mfge8. |
| Sensitivity |
1 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
MFGE8 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- Detection Antibody MFG-E8 (Item F): 2 vial of biotinylated anti-mouse MFG-E8 (each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 8 µl 20,000x concentrated HRP-conjugated streptavidin.
- MFG-E8 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse MFG-E8
- Standards (Item C): 2 vials of recombinant mouse MFG-E8.
- Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse MFG-E8 ELISA Kit (Colorimetric)
Background
Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for MFG-E8 ELISA Kit (KA1802). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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