Meteorin Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Meteorin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Meteorin isoform 1
  Gly22-Asp291
  Accession # Q8C1Q4
• Specificity: Detects mouse Meteorin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Metrn
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meteorin Antibody [Unconjugated]

• C16orf23
• c380A1.2
• Meteorin
• METRN

Background

Meteorin, named after meteor because this protein can transform glial cells into cells with an elongated tail, is a 30 kDa member of the Meteorin family (1). The secreted protein is also known as glial cell differentiation factor and hypoxia/reoxygenation regulatory factor. Mouse Meteorin is synthesized as a 291 amino acid (aa) precursor with a 21 aa signal sequence and a 270 aa mature chain.  Splicing variants produce two isoforms. Isoform 2 is missing residues 1‑164 found in isomer 1, and has a two aa substitution for aa 165‑166. Mouse Meteorin is 81% aa identical to human Meteorin (2). Meteorin is expressed in the central nervous system both during development and in adult mice (2). During development, Meteorin mRNA appears both in the central and peripheral nervous systems with the most prominent levels in neural progenitors, glial progenitors, and cells of the astrocyte lineage. In the adult mouse, Meteorin is detected only in the brain (2). Within the brain, the most prominent expression is found in the cerebellum where it is expressed by glial cells interspersed between Purkinje neurons (2). Meteorin is also detectable in several discrete neuronal populations, such as the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and various thalamic nuclei (2).Functionally, Meteorin plays important roles in both glial cell differentiation and axonal network formation during neurogenesis (1). In addition, research shows that when Meteorin is expressed and secreted by perivascular astrocytes, it upregulates thrombospondin-1/-2 to attenuate angiogenesis in the surrounding endothelial cells and to promote vascular maturation (3).

1. Nishino, J. et al. (2004) EMBO J. 23:1998.
2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. March 4 Published online.
3. Park, J.A. et al. (2008) Glia 56:247.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meteorin Antibody (AF3475)(1)

Publication using AF3475

• Seabrook TJ, Littlewood-Evans A, Brinkmann V, Pollinger B, Schnell C, Hiestand PC Angiogenesis is present in experimental autoimmune encephalomyelitis and pro-angiogenic factors are increased in multiple sclerosis lesions. J Neuroinflammation, 2010-12-22;7(0):95. 2010-12-22 [PMID: 21176212] (IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: Metrn
• Uniprot:
  • Q8C1Q4(Mouse)