MESDC2 Antibody - Azide and BSA Free

Western Blot: MESDC2 Antibody [NBP2-99668] - Anti-MESDC2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 25 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: MESDC2 Antibody [NBP2-99668] - MESDC2 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg NIH-3T3 Whole Cell Lysate. 2 ul anti-MESDC2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MESDC2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MESDC2 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human MESDC2 (Accession#: NP_055969.1; Ala 34-Lys 230)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MESD
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MESDC2 Antibody - Azide and BSA Free

• BOCA
• KIAA0081Renal carcinoma antigen NY-REN-61
• MESDC2
• MESDLDLR chaperone MESD
• mesoderm development candidate 2Mesoderm development protein

Background

Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway, since some LDLRs are coreceptors for the canonical Wnt pathway. Essential for specification of embryonic polarity and mesoderm induction

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MESD