Meprin alpha Subunit/MEP1A Antibody [Unconjugated]

Images

 
There are currently no images for Meprin alpha Subunit/MEP1A Antibody (AF3220).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Meprin alpha Subunit/MEP1A Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Meprin alpha Subunit/MEP1A
Val22-Gln601
Accession # AAA21338
Specificity
Detects human Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse MEP1A and recombinant human MEP1B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MEP1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 5
using
AF3220 in the following applications:

Publications
Read Publications using
AF3220 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin alpha Subunit/MEP1A Antibody [Unconjugated]

  • bA268F1.1 (meprin A alpha (PABA peptide hydrolase))
  • EC 3.4.24
  • EC 3.4.24.18
  • endopeptidase-2
  • MEP1A
  • meprin A subunit alpha
  • meprin A, alpha (PABA peptide hydrolase)
  • Meprin alpha Subunit
  • N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha
  • PABA peptide hydrolase
  • PPH alpha
  • PPHA

Background

Meprins are multimeric proteases composed of alpha and beta  subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha  subunits with or without beta  subunits) and Meprin B (composed of beta  subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 746 amino acid sequence of human meprin alpha subunit precursor consists of a signal peptide (residues 1 to 21), a pro region (residues 22 to 65), and a mature chain (residues 66 to 746) containing following domains, catalytic (residues 62 to 263), MAM (residues 264 to 433), MATH (residues 434 to 593), EGF-like (residues 670 to 710), transmembrane (residues 713 to 740), and cytoplasmic (residues 741 to 746). The pro enzyme terminating at residue 601 was expressed and the secreted protein purified from conditioned medium. The molecular masses of rhMEP1A are similar to those observed for the alpha  subunit of rat Meprin A (5).

  1. Bond, J.S. and Beynon, R.J. (1995) Protein Sci. 4:1247.
  2. Stocker, W. et al. (1995) Protein Sci. 4:823.
  3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
  4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.
  5. Bertenshaw, G.P. et al. (2003) J. Biol. Chem. 278:2522.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3300
Species: Mu
Applications: WB, IHC, IP
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-92546
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
H00003930-B01P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-67717
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-33903
Species: Hu
Applications: IHC, IHC-P
NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC
NB600-930
Species: Hu, Rt
Applications: WB, ELISA
NBP2-47559
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-89993
Species: Hu
Applications: WB, IHC, IHC-P
7270-IL/CF
Species: Hu
NBP1-90824
Species: Hu
Applications: WB, ICC/IF
NBP2-92677
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
AF1180
Species: Hu
Applications: WB, Simple Western, IHC, ICC

Publications for Meprin alpha Subunit/MEP1A Antibody (AF3220)(3)

Review for Meprin alpha Subunit/MEP1A Antibody (AF3220) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3220:
Filter by Applications
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 Meprin alpha Subunit/MEP1A AF3220
Enlarge
5
reviewed by:
Jessica Ong
Mouse 08/20/2019
View

Summary

Sample TestedC57BL/6J urine,C57BL/6J kidney
SpeciesMouse
LotXSH021907A

Comments

CommentsWestern blot 1:1000, IF 1:40

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Meprin alpha Subunit/MEP1A Antibody (AF3220) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF3220

Additional Meprin alpha Subunit/MEP1A Products

Bioinformatics Tool for Meprin alpha Subunit/MEP1A Antibody (AF3220)

Discover related pathways, diseases and genes to Meprin alpha Subunit/MEP1A Antibody (AF3220). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Meprin alpha Subunit/MEP1A Antibody (AF3220)

Discover more about diseases related to Meprin alpha Subunit/MEP1A Antibody (AF3220).
 

Pathways for Meprin alpha Subunit/MEP1A Antibody (AF3220)

View related products by pathway.

PTMs for Meprin alpha Subunit/MEP1A Antibody (AF3220)

Learn more about PTMs related to Meprin alpha Subunit/MEP1A Antibody (AF3220).

Blogs on Meprin alpha Subunit/MEP1A

There are no specific blogs for Meprin alpha Subunit/MEP1A, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Jessica Ong
08/20/2019
Application:
Species: Mouse

Bioinformatics

Gene Symbol MEP1A
Uniprot