Meprin alpha Subunit/MEP1A Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC, IP, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Meprin alpha Subunit/MEP1A Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Meprin alpha Subunit/MEP1A
  Val22-Gln601
  Accession # AAA21338
• Specificity: Detects human Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse MEP1A and recombinant human MEP1B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MEP1A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin alpha Subunit/MEP1A Antibody [Unconjugated]

• bA268F1.1 (meprin A alpha (PABA peptide hydrolase))
• EC 3.4.24
• EC 3.4.24.18
• endopeptidase-2
• MEP1A
• meprin A subunit alpha
• meprin A, alpha (PABA peptide hydrolase)
• Meprin alpha Subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha
• PABA peptide hydrolase
• PPH alpha
• PPHA

Background

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 746 amino acid sequence of human meprin alpha subunit precursor consists of a signal peptide (residues 1 to 21), a pro region (residues 22 to 65), and a mature chain (residues 66 to 746) containing following domains, catalytic (residues 62 to 263), MAM (residues 264 to 433), MATH (residues 434 to 593), EGF-like (residues 670 to 710), transmembrane (residues 713 to 740), and cytoplasmic (residues 741 to 746). The pro enzyme terminating at residue 601 was expressed and the secreted protein purified from conditioned medium. The molecular masses of rhMEP1A are similar to those observed for the alpha subunit of rat Meprin A (5).

1. Bond, J.S. and Beynon, R.J. (1995) Protein Sci. 4:1247.
2. Stocker, W. et al. (1995) Protein Sci. 4:823.
3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.
5. Bertenshaw, G.P. et al. (2003) J. Biol. Chem. 278:2522.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meprin alpha Subunit/MEP1A Antibody (AF3220)(4)

Publications using AF3220

• E Indersie, KB Hooks, C Capdeviell, M Fabre, N Dugot-Sena, A Desplat, S Lepreux, A Merched, CF Grosset, M Hagedorn Tracking cellular and molecular changes in a species-specific manner during experimental tumor progression in vivo Oncotarget, 2018-03-01;9(22):16149-16162. 2018-03-01 [PMID: 29662633] (IHC, Human)
• V Biasin, M Wygrecka, LM Marsh, C Becker-Pau, L Brcic, B Ghanim, W Klepetko, A Olschewski, G Kwapiszews Meprin ? contributes to collagen deposition in lung fibrosis Sci Rep, 2017-01-06;7(0):39969. 2017-01-06 [PMID: 28059112] (IHC-P, Mouse)
• Christian Herzog, Raju Marisiddaiah, Randy S. Haun, Gur P. Kaushal Basement membrane protein nidogen-1 is a target of meprin beta in cisplatin nephrotoxicity Toxicology Letters 2015-07-16 [PMID: 25957482]
• Herzog C, Haun R, Ludwig A, Shah S, Kaushal G ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A. J Biol Chem, 2014-03-24;289(19):13308-22. 2014-03-24 [PMID: 24662289] (Western Blot, Mouse)

Review for Meprin alpha Subunit/MEP1A Antibody (AF3220) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Jessica Ong Western blot and immunofluorescence Mouse 08/20/2019

Summary

• Application: Other
• Sample Tested: C57BL/6J kidney,C57BL/6J urine
• Species: Mouse
• Lot: XSH021907A

Comments

• Comments: Western blot 1:1000, IF 1:40

Bioinformatics

• Gene Symbol: MEP1A
• Uniprot:
  • AAA21338()