Meprin beta Subunit/MEP1B Antibody (289703) - Pro form

• Reactivity: Hu
• Applications: WB
• Clone: 289703
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Meprin beta Subunit/MEP1B Antibody (289703) - Pro form Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Meprin beta Subunit/MEP1B
  Thr23-Ser593
  Accession # Q16820
• Specificity: Detects human or mouse Pro Meprin beta Subunit/MEP1B in Western blots. It does not react with the mature form.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: MEP1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin beta Subunit/MEP1B Antibody (289703) - Pro form

• EC 3.4.24.63
• endopeptidase-2
• MEP1B
• meprin A subunit beta
• meprin A, beta
• Meprin B
• Meprin beta Subunit
• N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
• PABA peptide hydrolase
• PPH beta

Background

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of the human Meprin beta subunit precursor consists of a signal peptide (aa 1 - 21), a pro region (aa 22 - 61), and a mature chain (aa 62 - 701) containing the following domains: catalytic (aa 62 - 259), MAM (aa 260 - 429), MATH (aa 430 - 585), EGF-like (aa 604 - 644), transmembrane (aa 653 - 673), and cytoplasmic (aa 674 - 701).

1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
2. Stocker, W. et al. (1995) Protein Sci. 4:823.
3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MEP1B
• Uniprot:
  • Q16820()