Meprin beta Subunit/MEP1B Antibody (289703) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clone: 289703
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

Meprin beta Subunit/MEP1B Antibody (289703) [Alexa Fluor® 405] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Meprin β Subunit/MEP1B
  Thr23-Ser593
  Accession # Q16820
• Specificity: Detects human or mouse Pro Meprin beta Subunit/MEP1B in Western blots. It does not react with the mature form.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meprin beta Subunit/MEP1B Antibody (289703) [Alexa Fluor® 405]

• EC 3.4.24.63
• endopeptidase-2
• MEP1B
• meprin A subunit beta
• meprin A, beta
• Meprin B
• Meprin beta Subunit
• N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
• N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
• PABA peptide hydrolase
• PPH beta

Background

Meprins are multimeric proteases composed of alpha and beta  subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha  subunits with or without beta  subunits) and Meprin B (composed of beta  subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of the human Meprin beta subunit precursor consists of a signal peptide (aa 1 - 21), a pro region (aa 22 - 61), and a mature chain (aa 62 - 701) containing the following domains: catalytic (aa 62 - 259), MAM (aa 260 - 429), MATH (aa 430 - 585), EGF-like (aa 604 - 644), transmembrane (aa 653 - 673), and cytoplasmic (aa 674 - 701).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.